TABLE I

Kinetic Parameters for RSE Activity Change in LER Solutions Containing Citrate

Citrate (mM)	Polysorbate 20 (%)	pH	Temperature (°C)	NaCl (%)	RSE (EU/mL)	k	Y-intercept	r
0.5	0.05	7.4	23	0.8	5.0	0.98	−0.04	0.977
1	0.05	7.4	23	0.8	5.0	1.4	−0.09	0.970
2	0.05	7.4	23	0.8	5.0	2.3	−0.24	0.987
5	0.05	7.4	23	0.8	5.0	2.9	−0.06	0.995
10	0.05	7.4	2	0	0.2	0.048	−0.01	0.644
10	0.05	7.4	2	0	2.0	0.037	−0.07	0.657
10	0.05	7.4	23	0	5.0	4.6	−0.17	0.998
10	0.05	7.4	23	0.8	5.0	3.2	−0.15	0.998
10	0.2	7.4	23	0.8	5.0	3.4	−0.13	0.998
10	0.0001	7.4	23	0.8	5.0	0.077	−0.29	0.195
10	0.001	7.4	23	0.8	5.0	1.4	−0.17	0.981
10	0.01	7.4	23	0.8	5.0	3.2	−0.09	0.995
10	0.05	7.4	23	2.0	5.0	1.8	−0.07	0.984
10	0.05	7.4	23	3.0	5.0	0.71	−0.20	0.914
10	0.05	6.4	23	0	5.0	0.61	−0.31	0.930
10	0.05	6.9	23	0	5.0	0.93	−0.09	0.989
10	0.05	7.4	23	0	0.2	2.5	−0.15	0.990
10	0.05	7.4	23	0	0.2	3.5	−0.13	0.989
10	0.05	7.8	23	0	5.0	7.6	−0.04	0.996
10	0.05	7.4	30	0	2.0	9.3	−0.33	0.991
10	0.05	7.3	30	0	5.0	13	−0.31	0.993
10	0.05	7.4	36	0	2.0	32	−0.54	0.986
10	0.05	7.3	36	0	5.0	42	−0.11	0.999
25	0.05	7.4	23	0.7	5.0	2.9	−0.05	0.993

*Plotting incubation time versus the natural logarithm of reciprocals of endotoxin recovery provided the kinetic parameters, reaction rate constant k, y-intercept, and correlation coefficients r.