Polysorbate 80 Oxidation in Histidine Buffer in the Presence of Spiked Iron and Methionine1
| Fatty Acid | Comment | 5ppm Fe2 + Histidine + 1 mM Methionine | 5ppm Fe2 + Histidine |
|---|---|---|---|
| Oleic Acid | Intact (75% at T0) | 3.5 | 1.1 |
| Oleic Acid +2 [O]-2[H] | Oxidized | 2.6 | 3.1 |
| Oleic Acid +[O] | 6.7 | 6.7 | |
| Oleic Acid +[O]-2[H] | 17 | 13.3 |
↵1 Study conducted in glass vials.