Relationship between Cell Volume, Intrinsic Fluorescence, and the Number of Cells at the Time of Detection for Model Organisms
| Organism | Cell Volume (cubic microns) | Relative Volume per Cell | Fluorescein Equivalence per Cell | Relative Fluorescein per Cell | Number of Cells in a Micro-colony (scanning electron microscopy) | Number of Cells in a Micro-colony (eluted) | Fluorescein Equivalents at Time of Growth Direct Detection |
|---|---|---|---|---|---|---|---|
| C. albicans | 49 ± 5 | 5.6 | 3000 ± 1000 | 5.4 | 12 ± 2 | 8 ± 0.8 | 3.8 × 104 ± 1.5 × 104 |
| E. coli | 8.8 ± 0.7 | 1.0 | 560 ± 140 | 1 | 120 ± 20 | 170 ± 30 | 6.6 × 104 ± 3.2 × 104 |
| B. diminuta | 2.1 ± 0.4 | 0.23 | 81 ± 7 | 0.14 | 980 ± 290 | ND | 7.9 × 104 ± 2.6 × 104 |
ND = Not determined.