Results from a Distinguishable Cell Study Examining the Error Rate at Detecting 1 Cell/Well via Imaging for a Limiting Dilution Cloning Experiment
Total wells identified as 1 cell/well | 4403 |
Wells with outgrowth with 1 cell/well identified on day 0 | 2407 |
Outgrowth percentage | 54.7 |
Automated hitpicking wells with mixed population when only 1 cell identified on day 0 | 33 |
Manual verification wells with mixed population when only 1 cell identified on day 0 | 1 |
Corrected automated hitpicking error percentage | 2.7% |
Corrected manual verification error percentage | 0.09% |