TABLE III

Results from a Distinguishable Cell Study Examining the Error Rate at Detecting 1 Cell/Well via Imaging for a Limiting Dilution Cloning Experiment

 Total wells identified as 1 cell/well 4403 Wells with outgrowth with 1 cell/well identified on day 0 2407 Outgrowth percentage 54.7 Automated hitpicking wells with mixed population when only 1 cell identified on day 0 33 Manual verification wells with mixed population when only 1 cell identified on day 0 1 Corrected automated hitpicking error percentage 2.7% Corrected manual verification error percentage 0.09%