TABLE III

Results from a Distinguishable Cell Study Examining the Error Rate at Detecting 1 Cell/Well via Imaging for a Limiting Dilution Cloning Experiment

Total wells identified as 1 cell/well4403
Wells with outgrowth with 1 cell/well identified on day 02407
Outgrowth percentage54.7
Automated hitpicking wells with mixed population when only 1 cell identified on day 033
Manual verification wells with mixed population when only 1 cell identified on day 01
Corrected automated hitpicking error percentage2.7%
Corrected manual verification error percentage0.09%