Corrected Ghost Well Calculations for Seven Companies Examining Cloning Using Limiting Dilution or Flow Cytometry–Based Cloning
Company | A | B | C | D | E | F | G |
---|---|---|---|---|---|---|---|
Total Wells | 4224 | 1234 | 383 | 2304 | 46,080 | 3264 | 2304 |
No. of wells with ≥1 cell/well on day 0 | 1599 | 755 | 142 | 869 | 12,949 | 2221 | 633 |
No. of wells with outgrowth with ≥1 cell/well | 797 | 543 | 81 | 416 | 2947 | 913 | 620 |
Ghost well count | 49 | 31 | 6 | 5 | 261 | 32 | 34 |
Outgrowth % | 49.8 | 71.9 | 57.0 | 47.9 | 23.0 | 41.1 | 97.9 |
Corrected cloning ghost well % | 2.3 | 3.5 | 2.7 | 0.5 | 2.5 | 2.4 | 1.5 |
Corrected imager ghost well % | 5.8 | 5.4 | 6.9 | 1.2 | 8.1 | 3.4 | 5.2 |