TABLE I

Summary of Viral Clearance and Virus Carryover in Different Chromatography Modes

ModeResinVirusmAb AmAb B
LRVCarryoverLRVCarryover
AEX
B/E		NaïveMuLV
MVM
SV40		≥6.47
5.19
5.46		≥6.43
≥6.40
7.29		≥5.62
2.92
4.47		≥5.69
≥7.14
≥6.53
AgedMuLV
MVM
SV40		≥6.25
6.06
6.20		≥6.36
≥7.31
7.69		≥5.65
2.77
4.64		≥5.65
≥7.15
≥6.73
CEX
B/E		NaïveMuLV
MVM
SV40		1.99
0.90
1.86		≥6.49
≥6.52
≥7.14		No dataNo data
AgedMuLV
MVM
SV40		2.10
1.61
2.04		≥6.77
≥6.93
7.71		No dataNo data
MM
FT		NaïveMuLV
MVM
SV40		No dataNo data≥4.65
2.44
0.65		≥4.72
≥6.43
≥5.68
AgedMuLV
MVM
SV40		No dataNo data≥4.97
2.39
0.44		≥4.97
≥5.90
≥5.59
HI
mAb A: B/E
mAb C: FT		NaïveMuLV
MVM
SV40		3.20
0.97
0.54		≥6.13
≥5.88
7.05		≥5.14a
−0.09a
No data		No data
AgedMuLV
MVM
SV40		2.84
0.56
0.26		≥5.78
≥5.28
≥6.29		No dataNo data

  • B/E mode: bind and elute mode; FT mode: flow-through mode.

  • ↵a Data from mAb C, not mAb B.