Examples of Virus Removal by Anionic Retention
| Adsorber | Load (g/L) | Buffer System | LRV MuLV | LRV MVM | Remarks |
|---|---|---|---|---|---|
| Capto Q | 100 | 20 mM NaPi, 70 mM NaCl, pH 7.5 | ≥5.2 | 1.7/5.9 (PPV) | MuLV ↑, MVM →, PPV ↑ |
| 90 | 20 mM NaCitrate, pH 5.0 | ≥5.4 | 0.4 | With citrate: MuLV ↑, MVM ↓ | |
| Q Sepharose FF | 100 | 50 mM NaAc/Tris, pH 7.2 | 4.8 | 2.9 | – |
| Capto Adhere | 100 | Citrate, phosphate, Tris, pH 5.5 | ≥6.0 | 2.3 | With citrate: MuLV ↑, MVM → |
| Sartobind Q | 200 g/m2 500 g/m2 1000 g/m2 | Citrate, phosphate, Tris, pH 7.2 | ≥6.2 ≥6.2 ≥6.2 | 0.7 0.8 0.8 | With citrate: MuLV ↑, MVM ↓ |
| 240 g/m2 | 50 mM NaPi, pH 7.2 | ≥6.0 | 3.0/5.8 (PPV) | – | |
| 90ZB05A (30ZB + 90ZB) | 700 g/m2 1400 g/m2 | Citrate, phosphate, Tris, pH 5.5 | 2.9a 0.5a | 6.7 1.7a | Only at low load, with citrate MuLV ↑, MVM → |
| 30ZB | 350 g/m2 700 g/m2 1400 g/m2 | 2.6a 0.8a 0.5a | n/d | Only at very low load, with citrate MuLV ↑, no significant size exclusion | |
| Emphaze AEX HP | 700 g/m2 1400 g/m2 | 5.8 4.7a | 4.6 3.7a | With citrate: MuLV ↑, MVM ↑ no significant size exclusion without 0.2 μm membrane |
↵a LRV = (LRV at pH 5.5) − (LRV at pH 5.5 with 1 M NaCl).
Ion capacities: Emphaze AEX HP: 80 μEq/cm2 or 120 μEq/mL; Sartobind Q: 5 μEq/cm2 or 180 μEq/mL; Capto Q: 220 μEq/mL; Capto Adhere: 120 μEq/mL.