Dilution Series to Make the H2O2 Spike Solutions
| Dilution Sequence | Dilution Factor | H2O2 Concentration (ng/mL) | Previous Stock (μL) | Water or Protein (mL) | Total Solution (mL) |
|---|---|---|---|---|---|
| 0 | Stock solution | 353217829 | N/A | N/A | N/A |
| 1 | 100 | 3532178 | 10 | 0.99 | 1 |
| 2 | 100 | 35322 | 200 | 19.8 | 20 |
| 3 | 35.3 | 1000 | 7079 | 242.9 | 250 |
| Use of 1000 ng/mL solution for further dilution into final H2O2 samples | |||||
| Sample Number | Dilution Factor | H2O2 Concentration (ng/mL) | 1000 ng/mL H2O2 Solution (mL) | Water or Protein (mL) | Total Solution (mL) |
| 1 | 4 | 250 | 125 | 375 | 500 |
| 2 | 10 | 100 | 50 | 450 | 500 |
| 3 | 20 | 50 | 25 | 475 | 500 |