A new chromogenic endotoxin-specific assay using recombined limulus coagulation enzymes and its clinical applications

doi:10.1016/0009-8981(85)90273-6

Clinica Chimica Acta

Volume 149, Issue 1, 30 June 1985, Pages 55-65

https://doi.org/10.1016/0009-8981(85)90273-6 Get rights and content

Abstract

A conventional limulus test is not specific to endotoxin because of the presence in amebocyte lysate of a (1 → 3)-β-d-glucan-sensitive factor. By fractionating coagulation enzymes in the lysate and recombining only those factors involved in endotoxin-induced coagulation, we have developed a new test specific to endotoxin. The recombined enzymes reacted only with endotoxin, and not with fungal polysaccharides. Conventional amebocyte lysate, on the other hand, reacted with both of them. A good linearity was obtained with this method between endotoxin concentration and absorbance with a sensitivity of 1 pg/ml of Escherichia coli 0111:B4 endotoxin. The regression lines for different types of endotoxins were parallel to one another. For the correct diagnosis of endotoxemia, this new test has a definite advantage over the one using whole amebocyte lysate.

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Gelation of Limulus amebocyte lysate by an antitumor (1 → 3)-β-d-glucan
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Limulus anti-LPS factor: an anticoagulant which inhibits the endotoxin-mediated activation of Limulus coagulation system
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Citation Excerpt :
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Clinica Chimica Acta

Abstract

References (12)

Gelation of Limulus amebocyte lysate by an antitumor (1 → 3)-β-d-glucan

Biochem Biophys Res Commun

Limulus anti-LPS factor: an anticoagulant which inhibits the endotoxin-mediated activation of Limulus coagulation system

Biochem Biophys Res Commun

Clottable protein in Limulus: its localization and kinetics of its coagulation by endotoxin

Thromb Diath Haemorrh

Chromogenic substrates for horseshoe crab clotting enzyme

Haemostasis

A new (1 → 3)-β-d-glucan-mediated coagulation pathway found in Limulus amebocytes

FEBS Lett

Biochemical characterization of protein components associated with hemolymph coagulation system of limulus (Tachypleus tridentatus)

Thrombos Haemostas

Cited by (288)

Innate immune activation and antitumor effects of Lactobacillus-fermented Sparassis crispa extract in mice

Nonmolecular methods for the diagnosis of respiratory fungal infections

Interleukin-8 induces an elevation in the endotoxin activity assay (EAA) level: Does the EAA truly measure the endotoxin level?

Growth inhibition and apoptosis by an active component of OK-432, a streptococcal agent, via Toll-like receptor 4 in human head and neck cancer cell lines

Experimental application of a synthetic luminescent substrate assay using endotoxin-specific limulus amebocyte lysate to human blood

A potential test system for detecting contaminations by bacterial lipoproteins