1887

Abstract

Immunofluorescence microscopy-based identification of presumptive isolates, using the -specific mAb QUBPa3, revealed five organisms with an atypical cellular morphology. Unlike the coryneform morphology seen with types I and II, these isolates exhibited long slender filaments (which formed large tangled aggregates) not previously described in . No reaction with mAbs that label types IA (QUBPa1) and II (QUBPa2) was observed. Nucleotide sequencing of the 16S rRNA gene (1484 bp) revealed the isolates to have between 99.8 and 99.9 % identity to the 16S rRNA gene of the type IA, IB and II strains NCTC 737, KPA171202 and NCTC 10390, respectively. Analysis of the housekeeping gene (1047 bp) did reveal, however, a greater number of conserved nucleotide polymorphisms between the sequences from these isolates and those from NCTC 737 (98.9 % identity), KPA171202 (98.9 % identity) and NCTC 10390 (99.1 % identity). Phylogenetic investigations demonstrated that the isolates belong to a novel cluster or lineage distinct from types I and II. We now propose this new grouping as type III. The prevalence and clinical importance of this novel lineage amongst isolates of remains to be determined.

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2008-02-01
2024-03-28
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