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Comparison of UPLC and HPLC for Analysis of 12 Phthalates

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Abstract

Recent technological advances have resulted in the availability of reversed-phase chromatographic media of particle size 1.7 μm and a liquid-handling system that can be used to operate columns packed with these materials at much higher pressures. This technology, UPLC, has significant theoretical advantages in speed, resolution, and sensitivity of analysis, especially time saving and solvent consumption. The work discussed in this paper with new analytical method used for separation of 12 phthalates and the results were compared with those obtained by use of HPLC. Differences between the techniques, system suitability test data, and advantages and disadvantages of UPLC are discussed.

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References

  1. Waters Corporation (2004) Ultra performance LC by design. Waters Corporation USA, 720000880EN:LL. &LW-UL

  2. Waters Corporation (2004) Acquity ultra performance LC. Waters Corporation, USA, 720000820EN AG-UL

  3. Waters Corporation (2005) Acquity UPLC columns. Waters Corporation, 7200001140EN

  4. Ash MI (ed) (1989) Plasticizers, stabilizers and thickeners, Vol 3. Chemical Publishing, London

    Google Scholar 

  5. Fischer J, Ventura K, Prokeš B, Jandera P (1993) Chromatographia 37(1/2):47. doi:10.1007/BF02272187

    Article  CAS  Google Scholar 

  6. Castillo M, Oubiña A, Barceló D (1998) Environ Sci Technol 32:2180. doi:10.1021/es971042z

    Article  CAS  Google Scholar 

  7. Snell RP (1993) J AOAC Int 76:531

    CAS  Google Scholar 

  8. Dong MW, DiCesare JL (1982) J Chromatogr Sci 20:517

    CAS  Google Scholar 

  9. Silva MJ, Malek NA, Hodge CC, Reidy JA, Kato K, Barr DB et al (2003) J Chromatogr B Analyt Technol Biomed Life Sci 789:393. doi:10.1016/S1570-0232(03)00164-8

    Article  CAS  Google Scholar 

  10. Mitani K, Narimatsu S, Izushi F, Kataoka H (2003) J Pharm Biomed Anal 32:469. doi:10.1016/S0731-7085(03)00221-8

    Article  CAS  Google Scholar 

  11. Wahl HG, Hoffmann A, Haring HU, Liebich HM (1999) J Chromatogr A 847:1. doi:10.1016/S0021-9673(99)00138-7

    Article  CAS  Google Scholar 

  12. Castillo M, Barceló D, Pereira AS, Aquino Neto FR (1999) Trends Analyt Chem 18:26. doi:10.1016/S0165-9936(98)00066-1

    Article  CAS  Google Scholar 

  13. Nakamura Y, Oohata T, Tsuji H, Ito Y, Tatsuno T, Tomita I (1993) Nippon Hoso Gakkaishi 2:230

    CAS  Google Scholar 

  14. Peterson JH (1991) Food Addit Contam 8:701

    Google Scholar 

  15. Teirynck OA, Rosseel MT (1985) J Chromatogr A 342:399

    Google Scholar 

  16. Marin ML, Jimenez A, Lopez J, Vilaplana J (1996) J Chromatogr A 750:183. doi:10.1016/0021-9673(96)00393-7

    Article  CAS  Google Scholar 

  17. Li X, Zeng Z, Chen Y, Xu Y (2004) Talanta 63:1013. doi:10.1016/j.talanta.2004.01.006

    Article  CAS  Google Scholar 

  18. Petersen J, Breindahl T (2000) Food Addit Contam 17(2):133. doi:10.1080/026520300283487

    Article  CAS  Google Scholar 

  19. Ching NPH, Jahm GN, Subbarayan C, Bowen DV, Smit ALC Jr, Grossi CE et al (1981) J Chromatogr A 222:171

    CAS  Google Scholar 

  20. Sugita T, Hirayama K, Niino T, Ishibashi T, Yamada T (2001) Shokuhin Eiseigaku Zasshi 42:48. doi:10.3358/shokueishi.42.48 (J Food Hyg Soc Japan)

    Article  CAS  Google Scholar 

  21. Tsumura Y, Ishimitsu S, Nakamura Y, Yoshii K, Okuda M, Tonogai Y (2000) Shokuhin Eiseigaku Zasshi 41:254. doi:10.3358/shokueishi.41.254 (J Food Hyg Soc Japan)

    Article  CAS  Google Scholar 

  22. Sjoberg P, Bondesson U (1985) J Chromatogr A 344:167

    CAS  Google Scholar 

  23. Brumley WC, Shafter EM, Tillander PE (1994) J AOAC Int 77:1230

    CAS  Google Scholar 

  24. Earls AO, Axford IP, Braybrook JH (2003) J Chromatogr A 983:237. doi:10.1016/S0021-9673(02)01736-3

    Article  CAS  Google Scholar 

  25. Niino T, Ishibashi T, Itho T, Sakai S, Ishiwata H, Yamada T et al (2002) J Chromatogr B Analyt Technol Biomed Life Sci 780:35. doi:10.1016/S1570-0232(02)00413-0

    Article  CAS  Google Scholar 

  26. Shen H (2005) Talanta 66:734. doi:10.1016/j.talanta.2004.12.021

    Article  CAS  Google Scholar 

  27. Pharmacopoeia of the People’s Republic of China, China, 2005

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Acknowledgments

We acknowledge the Ministry of Science and Technology of the People’s Republic of China for financial support of this project (2006BAK10B03 and 2007JK015). Waters Corporation is also gratefully acknowledged for supplying the UPLC equipment and the Van Deemter plot in Fig. 1.

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Correspondence to Ting Wu.

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Wu, T., Wang, C., Wang, X. et al. Comparison of UPLC and HPLC for Analysis of 12 Phthalates. Chroma 68, 803–806 (2008). https://doi.org/10.1365/s10337-008-0788-y

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  • DOI: https://doi.org/10.1365/s10337-008-0788-y

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