Abstract
Recent technological advances have resulted in the availability of reversed-phase chromatographic media of particle size 1.7 μm and a liquid-handling system that can be used to operate columns packed with these materials at much higher pressures. This technology, UPLC, has significant theoretical advantages in speed, resolution, and sensitivity of analysis, especially time saving and solvent consumption. The work discussed in this paper with new analytical method used for separation of 12 phthalates and the results were compared with those obtained by use of HPLC. Differences between the techniques, system suitability test data, and advantages and disadvantages of UPLC are discussed.
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References
Waters Corporation (2004) Ultra performance LC by design. Waters Corporation USA, 720000880EN:LL. &LW-UL
Waters Corporation (2004) Acquity ultra performance LC. Waters Corporation, USA, 720000820EN AG-UL
Waters Corporation (2005) Acquity UPLC columns. Waters Corporation, 7200001140EN
Ash MI (ed) (1989) Plasticizers, stabilizers and thickeners, Vol 3. Chemical Publishing, London
Fischer J, Ventura K, Prokeš B, Jandera P (1993) Chromatographia 37(1/2):47. doi:10.1007/BF02272187
Castillo M, Oubiña A, Barceló D (1998) Environ Sci Technol 32:2180. doi:10.1021/es971042z
Snell RP (1993) J AOAC Int 76:531
Dong MW, DiCesare JL (1982) J Chromatogr Sci 20:517
Silva MJ, Malek NA, Hodge CC, Reidy JA, Kato K, Barr DB et al (2003) J Chromatogr B Analyt Technol Biomed Life Sci 789:393. doi:10.1016/S1570-0232(03)00164-8
Mitani K, Narimatsu S, Izushi F, Kataoka H (2003) J Pharm Biomed Anal 32:469. doi:10.1016/S0731-7085(03)00221-8
Wahl HG, Hoffmann A, Haring HU, Liebich HM (1999) J Chromatogr A 847:1. doi:10.1016/S0021-9673(99)00138-7
Castillo M, Barceló D, Pereira AS, Aquino Neto FR (1999) Trends Analyt Chem 18:26. doi:10.1016/S0165-9936(98)00066-1
Nakamura Y, Oohata T, Tsuji H, Ito Y, Tatsuno T, Tomita I (1993) Nippon Hoso Gakkaishi 2:230
Peterson JH (1991) Food Addit Contam 8:701
Teirynck OA, Rosseel MT (1985) J Chromatogr A 342:399
Marin ML, Jimenez A, Lopez J, Vilaplana J (1996) J Chromatogr A 750:183. doi:10.1016/0021-9673(96)00393-7
Li X, Zeng Z, Chen Y, Xu Y (2004) Talanta 63:1013. doi:10.1016/j.talanta.2004.01.006
Petersen J, Breindahl T (2000) Food Addit Contam 17(2):133. doi:10.1080/026520300283487
Ching NPH, Jahm GN, Subbarayan C, Bowen DV, Smit ALC Jr, Grossi CE et al (1981) J Chromatogr A 222:171
Sugita T, Hirayama K, Niino T, Ishibashi T, Yamada T (2001) Shokuhin Eiseigaku Zasshi 42:48. doi:10.3358/shokueishi.42.48 (J Food Hyg Soc Japan)
Tsumura Y, Ishimitsu S, Nakamura Y, Yoshii K, Okuda M, Tonogai Y (2000) Shokuhin Eiseigaku Zasshi 41:254. doi:10.3358/shokueishi.41.254 (J Food Hyg Soc Japan)
Sjoberg P, Bondesson U (1985) J Chromatogr A 344:167
Brumley WC, Shafter EM, Tillander PE (1994) J AOAC Int 77:1230
Earls AO, Axford IP, Braybrook JH (2003) J Chromatogr A 983:237. doi:10.1016/S0021-9673(02)01736-3
Niino T, Ishibashi T, Itho T, Sakai S, Ishiwata H, Yamada T et al (2002) J Chromatogr B Analyt Technol Biomed Life Sci 780:35. doi:10.1016/S1570-0232(02)00413-0
Shen H (2005) Talanta 66:734. doi:10.1016/j.talanta.2004.12.021
Pharmacopoeia of the People’s Republic of China, China, 2005
Acknowledgments
We acknowledge the Ministry of Science and Technology of the People’s Republic of China for financial support of this project (2006BAK10B03 and 2007JK015). Waters Corporation is also gratefully acknowledged for supplying the UPLC equipment and the Van Deemter plot in Fig. 1.
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Wu, T., Wang, C., Wang, X. et al. Comparison of UPLC and HPLC for Analysis of 12 Phthalates. Chroma 68, 803–806 (2008). https://doi.org/10.1365/s10337-008-0788-y
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DOI: https://doi.org/10.1365/s10337-008-0788-y