Reagentless detection of microorganisms by intrinsic fluorescence

Cory Estes; Andrew Duncan; Brad Wade; Christopher Lloyd; Walther Ellis Jr; Linda Powers

doi:10.1016/s0956-5663(03)00008-3

Reagentless detection of microorganisms by intrinsic fluorescence

Biosens Bioelectron. 2003 May;18(5-6):511-9. doi: 10.1016/s0956-5663(03)00008-3.

Authors

Cory Estes¹, Andrew Duncan, Brad Wade, Christopher Lloyd, Walther Ellis Jr, Linda Powers

Affiliation

¹ Department of Electrical Engineering, National Center for the Design of Molecular Function, Utah State University, Logan, UT 84322-4155, USA.

PMID: 12706557
DOI: 10.1016/s0956-5663(03)00008-3

Abstract

Quick and accurate detection of microbial contamination is accomplished by a unique combination of leading-edge technologies described in this and the accompanying paper. In this contribution, a hand-held prototype instrument is described which is capable of statistically sampling the environment for microbial contamination and determining cell viability. The technology is sensitive enough to detect very low levels ( approximately 20 cells/cm(2) or cm(3)) of microbes in seconds.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Validation Study

MeSH terms

Bacteria / isolation & purification*
Colony Count, Microbial / instrumentation*
Colony Count, Microbial / methods*
Electronics
Equipment Design
Equipment Failure Analysis
Feasibility Studies
Fungi / isolation & purification*
Indicators and Reagents
Miniaturization
Pilot Projects
Reproducibility of Results
Sensitivity and Specificity
Software
Spectrometry, Fluorescence / instrumentation*
Spectrometry, Fluorescence / methods*
Viruses / isolation & purification*

Substances

Indicators and Reagents