Fluorescence in situ hybridization using bacterial artificial chromosome (BAC) clones for the analysis of chromosome rearrangement in Chinese hamster ovary cells

Yihua Cao; Shuichi Kimura; Takayuki Itoi; Kohsuke Honda; Hisao Ohtake; Takeshi Omasa

doi:10.1016/j.ymeth.2011.11.002

Fluorescence in situ hybridization using bacterial artificial chromosome (BAC) clones for the analysis of chromosome rearrangement in Chinese hamster ovary cells

Methods. 2012 Mar;56(3):418-23. doi: 10.1016/j.ymeth.2011.11.002. Epub 2011 Nov 11.

Authors

Yihua Cao¹, Shuichi Kimura, Takayuki Itoi, Kohsuke Honda, Hisao Ohtake, Takeshi Omasa

Affiliation

¹ Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.

PMID: 22100493
DOI: 10.1016/j.ymeth.2011.11.002

Abstract

Chromosome identification using Chinese hamster ovary (CHO) genomic bacterial artificial chromosome (BAC) clones has the potential to contribute to the analysis and understanding of chromosomal instability of CHO cell lines and to improve our understanding of chromosome organization during the establishment of recombinant CHO cells. Fluorescence in situ hybridization imaging using BAC clones as probes (BAC-FISH) can provide valuable information for the identification of chromosomes. In this study, we identified chromosomes and analyzed the chromosome rearrangement in CHO cells using BAC-FISH methods.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
CHO Cells
Chromosome Aberrations*
Chromosomes, Artificial, Bacterial*
Cricetinae
Cricetulus
In Situ Hybridization, Fluorescence / methods*