Extracellular secretion of over 4 g·L −1 of the A33 scFv antibody fragment was achieved in
Pichia pastoris at the 10 L bioreactor scale using minimal medium and feedback control of the …

The expression of a humanized single-chain variable domain fragment antibody (A33scFv)
was optimized for Pichia pastoris with yields exceeding 4gL −1 . A33scFv recognizes a cell …

Extracellulor release of recombinant proteins simplifies later downstream steps for recovery
and purification. Of particular interest are antibodies and antibody fragments. In this thesis, I …

Glycoproteins could be highly sialylated, and controlling the sialic acid levels for some
therapeutic proteins is critical to ensure product consistency and efficacy. N‐acetylneuraminic …

Introduction. Pichia pastoris is methylotrophic yeast that can reach very high cell density
levels. P. pastoris has recently gained acceptance for the production of recombinant proteins, …

Air–liquid interface rings were observed on the side walls of stainless steel buffer vessels
after certain downstream buffer preparations. Those rings were resistant to regular cleaning-in-…

Biologics drug substance is ideally stored and transported in a frozen state to minimize
potential temperature related instabilities. Currently, BMS utilizes compressor-based freezers or …

Increasing clinical and industrial advances in the pharmaceutical world requires technological
improvements in cell culture development and monoclonal antibody (mAb) production. …