Highlights • ‘Behind-the-scenes’ look into the development of Pfizer–BioNTech’s COVID-19
vaccine. • Description of how Pfizer leveraged decades of developed platforms to enable …

Recently it has been established that low molecular weight displacers can be successfully
employed for the purification of proteins in hydrophobic interaction chromatography (HIC) …

Displacement chromatography of proteins was successfully carried out in both hydrophobic
interaction and reversed‐phase chromatographic systems using low‐molecular weight …

In recent years, most pharmaceutical companies have focused on the development of
monoclonal antibodies (mAbs). Increasing upstream titers and shrinking development timelines …

Depth filtration is employed in the production of many biological products. Although high
throughput process development is available for most purification operations, options for high …

The phrase “Process is the Product” is often applied to biologics, including multicomponent
vaccines composed of complex components that evade complete characterization. Vaccine …

The purpose of this work was the chemical characterization of variants of the recombinant
human brain derived neurotrophic factor (rHu-BDNF), expressed in Escherichia coli. This …

This work investigates the utility of RPLC displacement chromatography for the purification
of recombinant brain derived neurotrophic factor (rHu‐BDNF) from its variants and E. coli.…

The biotech industry is under increasing pressure to decrease both time to market and
development costs. Simultaneously, regulators are expecting increased process understanding. …

Multivalent polysaccharide conjugate vaccines are typically comprised of several different
polysaccharides produced with distinct and complex production processes. Particle …