Characterization and Molecular Analysis of Macrolide-Resistant Mycoplasma pneumoniae Clinical Isolates Obtained in Japan

…, T Kenri, Y Sasaki, A Horino, M Shintani… - Antimicrobial agents …, 2004 - Am Soc Microbiol
In recent years, Mycoplasma pneumoniae strains that are clinically resistant to macrolide
antibiotics have occasionally been encountered in Japan. Of 76 strains of M. pneumoniae …

Epidemiological study of Mycoplasma pneumoniae infections in Japan based on PCR-restriction fragment length polymorphism of the P1 cytadhesin gene

…, M Iseki, R Yamashita, M Shintani… - Journal of clinical …, 1996 - Am Soc Microbiol
Two hundred fifty strains of Mycoplasma pneumoniae isolated during the past 20 years in
Japan were classified into two groups (I and II) based upon different PCR-restriction fragment …

Evaluation of a new method for identification of bacteria based on sequence homology of 16S rRNA gene

T Sasaki, T Nishiyama, M Shintani… - PDA Journal of …, 1997 - journal.pda.org
A new identification method for bacteria based on partial sequences of divergent regions of
the 16S rRNA gene was evaluated. The method involves PCR-based amplification of 16S …

Application of PCR for detection of mycoplasma DNA and pestivirus RNA in human live viral vaccines

T Sasaki, R Harasawa, M Shintani, H Fujiwara… - Biologicals, 1996 - Elsevier
PCR techniques were applied for the detection of mycoplasma DNA and pestivirus RNA to 43
lots of live viral vaccines (measles, mumps, rubella, and oral poliomyelitis) produced by six …

Detection and discrimination of Mycoplasma pneumoniae and Mycoplasma genitalium by the in vitro DNA amplification

Y Sasaki, M Shintani, T Shimada… - Microbiology and …, 1992 - jstage.jst.go.jp
MATERIALS AND METHODS Organisms and culture methods. As human related mycoplasmas,
M. pneumoniae strains(Mac, 104166, M129, and five clinical isolates), M. genitalium …

Inhibition of growth of mammalian cell cultures by extracts of arginine-utilizing mycoplasmas

T Sasaki, M Shintani, K Kihara - In vitro, 1984 - Springer
Mechanisms of the inhibition of growth of mammalian cell cultures caused by mycoplasmal
infection were investigated by using cell-free extracts of 14 species of mycoplasmas. In four …

Comparison of the Efficacy of Egg Yolk Extract and Horse Serum for Growth of Mycoplasma pneumoniae

T Sasaki, M Shintani, K Kihara - Microbiology and immunology, 1985 - Wiley Online Library
The usefulness of chicken egg yolk extract as a substitute for horse serum in culture media
for Mycoplasma pneumoniae was investigated. As a growth‐supporting factor in the growth …

Attachment of Mycoplasma hominis and M. orale to Human Diploid Lung Fibroblasts

…, K Yamada, T Sasaki, M Shintani… - Microbiology and …, 1981 - Wiley Online Library
The process of attachment of Mycoplasma hominis and M. orale to HAIN‐55 cells, derived
from normal embryonic human lung, was investigated quantitatively. The attachment reached …

Utility of egg yolk medium for cultivation of Mycoplasma pneumoniae

T Sasaki, M Shintani, K Kihara - Journal of clinical microbiology, 1983 - Am Soc Microbiol
Chicken egg yolk broth medium containing 10% (vol/vol) phosphate-buffered saline extract
of egg yolk instead of horse serum showed better growth-promoting activity for Mycoplasma …

Behavior of Mycoplasma hominis in a Human Diploid Cell Culture System

T Sasaki, M Shintani, K Yamada… - Microbiology and …, 1981 - Wiley Online Library
The behavior of Mycoplasma hominis in normal human embryonic lung fibroblast (HAIN‐55)
cell cultures was investigated. Multiplication patterns of cell‐associated mycoplasmas and …