Cell culture and gene transcription effects of copper sulfate on Chinese hamster ovary cells
Y Qian, SF Khattak, Z Xing, A He… - Biotechnology …, 2011 - Wiley Online Library
This study reports the effects of varying concentrations of copper sulfate on the metabolic
and gene transcriptional profile of a recombinant Chinese hamster ovary (CHO) cell line …
and gene transcriptional profile of a recombinant Chinese hamster ovary (CHO) cell line …
Improving titer while maintaining quality of final formulated drug substance via optimization of CHO cell culture conditions in low-iron chemically defined media
During biopharmaceutical process development, it is important to improve titer to reduce
drug manufacturing costs and to deliver comparable quality attributes of therapeutic proteins, …
drug manufacturing costs and to deliver comparable quality attributes of therapeutic proteins, …
Mitogen-activated protein kinase activation resulting from selective oncogene expression in NIH 3T3 and rat 1a cells.
…, SK Gupta, LE Heasley, NX Qian… - Proceedings of the …, 1992 - National Acad Sciences
Mitogen-activated protein kinases (MAPKs) are serine/threonine kinases that are rapidly
activated in response to a variety of growth factors in many cell types. MAPKs are activated by …
activated in response to a variety of growth factors in many cell types. MAPKs are activated by …
Brown drug substance color investigation in cell culture manufacturing using chemically defined media: A case study
Drug substance (DS) color is an important quality attribute for release, stability and comparability
studies of biologics. With the increase of DS concentrations and biologics pipelines …
studies of biologics. With the increase of DS concentrations and biologics pipelines …
Distinct role of ZAP-70 and Src homology 2 domain-containing leukocyte protein of 76 kDa in the prolonged activation of extracellular signal-regulated protein kinase …
…, TD Humphreys, A Kumar, NX Qian… - The Journal of …, 2003 - journals.aai.org
Stimulation of T lymphocytes with the ligand for the CXCR4 chemokine receptor stromal cell-derived
factor-1α (SDF-1α/CXCL12), results in prolonged activation of the extracellular …
factor-1α (SDF-1α/CXCL12), results in prolonged activation of the extracellular …
Epitope-tagged Gq alpha subunits: expression of GTPase-deficient alpha subunits persistently stimulates phosphatidylinositol-specific phospholipase C but not …
NX Qian, S Winitz, GL Johnson - Proceedings of the …, 1993 - National Acad Sciences
Gq is the heterotrimeric guanine nucleotide-binding protein that activates the beta isoforms
of phosphatidyl-inositol-specific phospholipase C (PI-PLC). The Gq alpha-subunit …
of phosphatidyl-inositol-specific phospholipase C (PI-PLC). The Gq alpha-subunit …
Stromal cell-derived factor-1 signaling via the CXCR4-TCR heterodimer requires phospholipase C-β3 and phospholipase C-γ1 for distinct cellular responses
…, AG Miamen, AO Bamidele, NX Qian… - The Journal of …, 2011 - journals.aai.org
The CXCR4 chemokine receptor is a G protein-coupled receptor that signals in T lymphocytes
by forming a heterodimer with the TCR. CXCR4 and TCR functions are consequently …
by forming a heterodimer with the TCR. CXCR4 and TCR functions are consequently …
[HTML][HTML] Expression of a mutant Gi2 alpha subunit inhibits ATP and thrombin stimulation of cytoplasmic phospholipase A2-mediated arachidonic acid release …
S Winitz, SK Gupta, NX Qian, LE Heasley… - Journal of Biological …, 1994 - Elsevier
The 85-kDa cytoplasmic phospholipase A2 (cPLA2) is the major hormone and growth factor-regulated
enzyme that catalyzes release of arachidonic acid in mammalian cells. Activation …
enzyme that catalyzes release of arachidonic acid in mammalian cells. Activation …
Hypoxia influences protein transport and epigenetic repression of CHO cell cultures in shake flasks
Shake flasks and bench‐top bioreactors are widely used for cell culture process development,
however, culture performances significantly differ between them. In order to apply the …
however, culture performances significantly differ between them. In order to apply the …
[HTML][HTML] Improved titer in late-stage mammalian cell culture manufacturing by re-cloning
…, K Bierilo, G Tremml, G Rizzi, EF Langsdorf, NX Qian… - Bioengineering, 2022 - mdpi.com
Improving productivity to reduce the cost of biologics manufacturing and ensure that therapeutics
can reach more patients remains a major challenge faced by the biopharmaceutical …
can reach more patients remains a major challenge faced by the biopharmaceutical …