How pH, temperature, and time of incubation affect false-positive responses and uncertainty of the LAL gel-clot test

FR Lourenço, TDS Botelho… - PDA Journal of …, 2012 - journal.pda.org
The limulus amebocyte lysate (LAL) test is the simplest and most widely used procedure for
detection of endotoxin in parenteral drugs. The LAL test demands optimal pH, ionic strength, …

Evaluating the potential, applicability, and effectiveness of ozone sterilization process for medical devices

T de Souza Botelho-Almeida, FR Lourenço… - Journal of …, 2018 - Springer
Purpose Ozone (O 3 ) can be considered the most potent natural germicide against microorganisms
(in vegetative and spore forms) with high efficiency and speed, because of its highly …

[HTML][HTML] Ozonated water in disinfection of hospital instrument table

…, GV Schmitz, T de Souza Botelho Almeida… - Research on Biomedical …, 2023 - Springer
Purpose The walls, ceiling, and floor of a surgical environment, as well as the surfaces used
in this place, must be submitted to a disinfection protocol to minimize nosocomial infections. …

Disinfection of human amniotic membrane using a hydrodynamic system with ozonated water

…, HC Carvalho, T de Souza Botelho… - Ozone: Science & …, 2023 - Taylor & Francis
Human amniotic membrane (hAM) has a great potential in regenerative medicine as it can
be disinfected, preserved, and stored. The present study evaluated a hydrodynamic system …

Evaluation of the Ozone Effects on Human Hair Fiber: A Preliminary In Vitro Study

…, H Braga Carneiro, T de Souza Botelho… - Ozone: Science & …, 2023 - Taylor & Francis
Research with ozone has shown potential for applicability, including use of ozone as an
environmentally friendly alternative to personal care products (PCPs). However, for hair, there …

Inactivation of Staphylococcus aureus in Surgical Needles by Exposure to Ozone Gas and Low Pressure

…, HC Carvalho, T de Souza Botelho… - Ozone: Science & …, 2023 - Taylor & Francis
Ethylene oxide and gamma irradiation are two well-established sterilizing processes for
surgical materials; however, they present toxicity and high cost, respectively. Ozone Gas, O 3(g) …

Doseamento microbiológico de vancomicina-desenvolvimento e validação de método empregando leitura cinética em microplacas

TS Botelho - 2011 - teses.usp.br
O ensaio turbidimétrico tem como princípio a redução da densidade óptica em suspensões
microbianas, decorrente o incremento na concentração do antibiótico. No ensaio …

[PDF][PDF] Doseamento microbiológico de vancomicina–desenvolvimento e validação de método empregando leitura cinética em microplacas

T de Souza Botelho - 2010 - scholar.archive.org
O ensaio turbidimétrico tem como princípio a redução da densidade óptica em suspensões
microbianas, decorrente o incremento na concentração do antibiótico. No ensaio …

Avaliação da eficácia e aplicabilidade de processo de esterilização por ozônio

TS Botelho-Almeida - 2017 - teses.usp.br
A busca contínua por tecnologias de esterilização a baixa temperatura deve-se à necessidade
de adequação dos agentes esterilizantes às características físicoquímicas dos produtos, …

Vancomycin Microbial Assay Using Kinetic-Reading Microplate System

T de Souza Botelho… - Current Pharmaceutical …, 2013 - ingentaconnect.com
The aim of this work was to develop a microbiological assay of vancomycin employing
microplates and kinetic reading mode, and to validate the developed method by assessing of …