Bacterial cell stock paste of Brevundimonas diminuta (American Type Culture Collection#
19146) used for filter validation was evaluated by standard microbiological procedures to …

Brevundimonas diminuta (ATCC 19146) is a standard organism for validation of sterilizing-
grade membrane filters. Cell size is critical for the determination of retention characteristics …

Microbial challenge testing is a common procedure to determine the retention efficiency,
performance, and validity of a sterilizing-grade filter. The ASTM 838-05 standard describes a …

The ASTM 838-05 standard describes a bacteria challenge test procedure based on
Brevundimonas diminuta (ATCC 19146) to verify a 0.2 μm rated sterilizing-grade filter. For …

Brevundimonas diminuta is the organism most commonly used for challenge testing of
sterilizing-grade filter membranes. ASTM F838-05 and PDA Technical Report 26 rely on B …

Approximately 97% of filter validation tests result in the demonstration of absolute retention
of the test bacteria, and thus sterile filter validation failure is rare. However, while …

The results of scanning electron microscopic (SEM) and fatty acid methyl ester (FAME)
characterization of the bacterial species shown to penetrate conventional 0.2/0.22 μm rated …

While conducting long term bacteria challenge tests of 0.2-µ. m rated sterilizing grade filters
which retained 100% of the standard challenge organism P. diminuta, it was discovered that …

Although 0.1 μm rated filters intended for pharmaceutical sterilization applications have
been commercially available for at least 15 years, there is no industry-wide standard for …

Brevundimonas diminuta is a small Gram-negative bacterium used for validation of
membranes and filters used in the pharmaceutical and drinking water treatment industries …