The paper describes considerations taken with regard to a recombinant human cell line
(HEK293) producing a therapeutic protein. When working with HEK293 cells, besides …

Recombinant DNA technology as well as hybridoma technology provide an outstanding
opportunity for the preparation of complex human proteins with significant impact on …

Various number of expression and host systems are used in biologics manufacturing. The
most commonly preferred systems are based on bacteria, yeast, mammalian cells, insect …

Biotherapeutic proteins represent a mainstay of treatment for a multitude of conditions, for
example, autoimmune disorders, hematologic disorders, hormonal dysregulation, cancers …

Murine monoclonal antibodies are now routinely produced from highly expressing
hybridoma cell lines for human clinical applications. Epstein Barr Virus transformed human …

Highlights•Manufacture of biopharmaceuticals, virus-like particles and viral vectors.•System
biology,'omics, cellular phenotype and cell engineering.•Cell line development, culture and …

Throughout the history of biologics administered for human use, one can find numerous
examples of products that have been contaminated with potential hu-man pathogens. The …

Huddinge University Hospital is a major teaching hospital affiliated with the Karolinska
Institute in Southern Stockholm. For the past few years several groups have been working …

Mammalian cell cultures are increasingly used for the production of complex
biopharmaceuticals including viral vectors and vaccines. HEK293 is the predominant cell …