Of importance to the biological properties of proteins produced in cell culture systems are
the complex post-translational modifications that are affected by variations in process …

BACKGROUND The enormous success of antibody therapeutics has resulted in attempts to
enhance protein expression which requisites high energy demand which leads to oxidative …

<? xm-replace_text {Heading}?> Abstract The manufacturing of a biologic drug from
mammalian cells results in not a single substance, but an array of product isoforms, also …

Since the introduction of recombinant human insulin nearly two decades ago, recombinant
proteins have increasingly been utilized as therapeutic agents. In addition, expression of …

Chinese hamster ovary (CHO) cells are widely used for the manufacture of therapeutic mAbs
with high productivity processes generating 5–10 g/L mAb. High productivity processes …

Protein oxidation is defined here as the covalent modification of a protein induced either
directly by reactive oxygen species or indirectly by reaction with secondary by-products of …

Measuring and monitoring of protein oxidation modifications is important for
biopharmaceutical process development and stability assessment during long-term storage …

Oxidation is one of the major chemical degradation pathways for protein pharmaceuticals.
Methionine, cysteine, histidine, tryptophan, and tyrosine are the amino acid residues most …

Oxidation is a common degradation pathway that affects therapeutic proteins and peptides
during production, purification, formulation, transportation, storage and handling of solid and …

Elevation of lactate, ammonia, osmolality, and carbon dioxide to inhibitory levels was
reported to have adverse effects on cell growth and protein productivity in mammalian cell …