Protection of a model protein, lactate dehydrogenase, by encapsulation in liposome

S Nema, KE Avis - PDA Journal of Pharmaceutical Science and …, 1996 - journal.pda.org
The objective of this study was to determine if encapsulating a model protein, lactate
dehydrogenase (LDH), inside liposomes can protect it during storage, upon freeze-thaw …

Protection of lactate dehydrogenase against protease digestion by encapsulation in liposomes

S Nema, DM Kiick, KE Avis - PDA journal of Pharmaceutical Science …, 1994 - journal.pda.org
Lactate dehydrogenase (LDH) was entrapped in phosphatidylcholine liposomes to evaluate
the protective effect of liposomes against protease digestion. Three different preparations of …

[HTML][HTML] Protection mechanisms of excipients on lactate dehydrogenase during freeze-thawing and lyophilization

Y Mi - 2002 - dc.uthsc.edu
Problem-The primary objective of this dissertation was to elucidate the protective effects and
mechanisms of excipients on a labile model protein, lactate dehydrogenase (LDH), during …

Increasing the entrapment of protein-loaded liposomes with a modified freeze–thaw technique: a preliminary experimental study

YZ Zhao, CT Lu - Drug development and industrial pharmacy, 2009 - Taylor & Francis
The objective of this study was to investigate the possibility of increasing the entrapment of
protein-loaded liposomes with a modified freeze–thaw technique. Blank liposomes were …

The protective effect of free and membrane-bound cryoprotectants during freezing and freeze-drying of liposomes

M Ausborn, H Schreier, G Brezesinski, H Fabian… - Journal of controlled …, 1994 - Elsevier
Liposomes were prepared from natural (EPC) and hydrogenated (HEPC) egg
phosphatidylcholine, with and without cholesterol (CHOL), from sucrose fatty acid ester …

Stability of protein-encapsulating DRV liposomes after freeze-drying: a study with BSA and t-PA

V Ntimenou, S Mourtas… - Journal of liposome …, 2006 - Taylor & Francis
Stability of protein-encapsulating DRV (dried-rehydrated vesicle) liposomes is evaluated
after freeze-drying vesicles in presence (or not) of trehalose. Two proteins, bovine serum …

Lyophilization of liposomal drug forms

OY Arshinova, EV Sanarova, AV Lantsova… - Pharmaceutical …, 2012 - Springer
Lyophilization is a promising approach to ensuring the long-term stability of liposomes.
Decades of studies in this field have revealed various cryoprotective mechanisms such as …

Freeze-drying of liposomes: theory and practice

ECA van Winden - Methods in enzymology, 2003 - Elsevier
Freeze-drying of liposomes can prevent hydrolysis of the phospholipids and physical
degradation of the vesicles during storage. In addition, it may help stabilize the substance …

Freeze-thaw studies of a model protein, lactate dehydrogenase, in the presence of cryoprotectants

S Nema, EA Kenneth - PDA Journal of Pharmaceutical Science and …, 1993 - journal.pda.org
The objective of this study was to investigate the behavior of lactate dehydrogenase (LDH).
upon freezing and thawing, alone or in the presence of several selected cryoprotectants …

Protection mechanism of Tween 80 during freeze–thawing of a model protein, LDH

A Hillgren, J Lindgren, M Aldén - International Journal of Pharmaceutics, 2002 - Elsevier
The purpose of the study was to investigate the protective mechanism of a non-ionic
surfactant, Tween 80, at freeze–thawing with controlled temperature history of a model …