Effect of steam-heat treatment with/without divalent cations on the inactivation of lipopolysaccharides from several bacterial species

T Bamba, R Matsui, K Watabe - PDA Journal of Pharmaceutical …, 1996 - journal.pda.org
The inactivation of endotoxin from six species of smooth gram-negative bacteria (S-form) by
steam-heat treatment was investigated using the Limulus amebocyte lysate (LAL) assay …

Wet heat inactivation of lipopolysaccharide from E. coli serotype 055: B5

S Fujii, M Takai, T Maki - PDA Journal of Pharmaceutical Science …, 2002 - journal.pda.org
Wet heat inactivation of lipopolysaccharide (LPS) from E. coli Serotype 055: B5 was
investigated. The LPS solutions were heated at study temperatures ranging from 78° C to …

Destruction of typical endotoxins by dry heat as determined using LAL assay and pyrogen assay

T Nakata - PDA Journal of Pharmaceutical Science and …, 1993 - journal.pda.org
The kinetics of destruction by dry heat of two typical endotoxins, Escherichia coli 055: B5 (E.
coli endotoxin) and Salmonella abortus equi (S. abortus equi endotoxin), were determined …

Studies on the thermal destruction of Escherichia coli endotoxin

KE Avis, RC Jewell, JD Ludwig - PDA Journal of Pharmaceutical …, 1987 - journal.pda.org
The dry heat destruction of Escherichia coli endotoxin in Type I glass vials was studied at
three oven operating temperatures, namely, 175° C, 210° C, and 250° C, utilizing typical …

Inactivation of Escherichia coli Endotoxin by Soft Hydrothermal Processing

T Miyamoto, S Okano, N Kasai - Applied and environmental …, 2009 - Am Soc Microbiol
Bacterial endotoxins, also known as lipopolysaccharides, are a fever-producing by-product
of gram-negative bacteria commonly known as pyrogens. It is essential to remove …

Hypothermia enhances the biological activity of lipopolysaccharide by altering its fluidity state

J Luhm, AB Schromm, U Seydel… - European journal of …, 1998 - Wiley Online Library
Lipopolysaccharides (LPS, endotoxin) of Gram‐negative bacteria are among the main
causes of sepsis and septic shock. In the present study, the influence of temperature on the …

Destruction of challenged endotoxin in a dry heat oven

T Nakata - PDA Journal of Pharmaceutical Science and …, 1994 - journal.pda.org
The amount and pyrogenicity of challenged endotoxin (derived from E. coli 055: B5, 10,000
endotoxin units, EU) processed in a dry heat oven at 200° and 250° C was measured and …

The heat sensitivity of cytokine-inducing effect of lipopolysaccharide

B Gao, Y Wang, MF Tsan - Journal of leukocyte biology, 2006 - academic.oup.com
Heat inactivation by boiling has been widely used as a criterion to determine whether the
observed effects of a protein preparation are a result of lipopolysaccharide (LPS) …

Physicochemical and biological characterizations of complex formation between lipopolysaccharide and lysozyme

K Takada, N Ohno, T Yadomae - Journal of Endotoxin …, 1995 - journals.sagepub.com
Endotoxin (lipopolysaccharide, LPS) from Gram-negative bacteria induces a variety of
biological effects in mammals. We have earlier demonstrated that lysozyme (LZM) will bind …

Interaction between lipopolysaccharide and detergents detected by differential scanning calorimetry

D Lörinczy, B Kocsis - Thermochimica acta, 2001 - Elsevier
The Gram-negative bacteria can cause very serious diseases. One of their pathogenetic
factors is the endotoxic lipopolysaccharide (LPS). This molecule is a component of outer …