The purpose of this paper is to explore the application and mechanisms of polyethylene
glycol 8000 (PEG 8000) on stabilizing lactate dehydrogenase (LDH) during lyophilization. In …

Lactate dehydrogenase (LDH) is a tetrameric enzyme that has been used as a model for
labile protein drugs. Polyethylene glycols (PEGs) have been proposed as excipients to …

The purpose of this study was to explore the cryoprotection mechanisms of high molecular
weight polyethylene glycols (PEGs)(eg, PEG 4000 and PEG 8000) on lactate …

The protection of lactate dehydrogenase (LDH) by low concentrations of the non-surface-
active polyethylene glycol (PEG 6000) or the non-ionic surfactant PEG dodecyl ether (Brij …

Purpose In order to understand the stabilizing effects of disaccharides on freeze-dried
proteins, the enzymatic activity of lactate dehydrogenase (LDH) formulations containing four …

The stresses during the secondary‐drying stage of lyophilization were investigated using a
controlled humidity mini‐freeze‐dryer [Luthra S, Obert J‐P, Kalonia DS, Pikal MJ. 2007 …

Freeze-drying of protein formulations is frequently used to maintain protein activity during
storage. The freeze-drying process usually requires long primary drying times because the …

The effects of eight cyclodextrins (CDs) on the stability of freeze‐dried lactate
dehydrogenase (LDH) were investigated. Five low‐molecular weight saccharides were used …

Purpose. The stabilizing effect of amphiphilic excipients and sugars against protein
inactivation during freeze-drying was studied in relation to their physical states in freeze …

The objective of this study was to investigate both the cryoprotective and lyoprotective effects
of the polymer hydroxyethyl cellulose (HEC) on the model protein lactate dehydrogenase …