Factors Affecting Recovery of Neosartorya fischeri Ascospores after Exposure to Dry Heat

Abstract

Recovery of Neosartorya fischeri ascospores subjected to a dry heat treatment (DHT) at 95°C, 50% relative humidity (RH) for 60 minutes increased exponentially as the initial temperature of the recovery buffer increased. Different diluents were evaluated and the same recovery pattern was obtained when water or dilute buffers were used to recover the DHT spores. However, when glycerol was added to the buffer, the number of spores recovered in solutions held in ice water increased with increasing glycerol concentration. When the DHT spores were exposed to an atmosphere saturated with water vapor (100% RH) before being placed in the buffer, the recovery was independent of the initial temperature of the buffer. This occurred even if the spores were subsequently dried before being introduced into the buffer. It is hypothesized that the temperature-dependent recovery was due to injury of the DHT spores during the sudden rehydration in dilute solutions at low temperatures.