Resistance of Neosartorya fischeri to Wet and Dry Heat

Abstract

ABSTRACT: Dry heat resistance parameters for Neosartorya fischeri ascospores were obtained at 90°C and 95°C under 30%, 40%, 50%, 60%, and 75% relative humidity (RH) conditions. The dry heat treated spores were exposed to saturated water vapor (for 20–24 h at 4°C) prior to recovery in buffer held at two temperatures (0°C and 80°C). Approximately the same level of recovery was obtained at the two buffer temperatures except at the shortest heating times for the heat treatment carried at 30% and 40% RH, where the number of survivors was significantly higher for spores placed in the buffer held at 80°C. The effect of this high temperature was attributed to heat activation of the ascospores that remained dormant during the dry heat treatment conditions mentioned above. The wet heat resistance of N. fischeri ascospores was also determined at temperatures ranging from 82.5°C to 95°C. The results indicate that as the RH decreased, the heat resistance of the ascospores increased. There were about four orders of magnitude difference in the heat resistance between wet heat (100% RH) and the lowest dry heat treatment condition (30% RH).