Moist thermal resistance parameters of spores of B. stearothermophilus ATCC 7953, inoculated in aqueous suspensions and on strips, were evaluated at 118°C and 121°C, by the proposed serum-bottle-mini-autoclave method. The present work studied the effect of alkaline calcium exchange-inducing storage treatments on the viability and moist heat-resistance of B. stearothermophilus spores, stored at – 18°C, following impregnation into filter paper strips which had been previously treated by immersion in alkaline 0.02 M calcium acetate. The thermal resistance of B. stearothermophilus spores on strip at set work conditions as biological indicator exhibited an average medium D (121°C) of 1.92 min for concentrations of 105–106 spores per strip over 360 days of storage in a freezer. The serum-bottle-mini-autoclave measuring system resulted in reasonably accurate and reproducible Bls calibration, before using them in an equipment validation program. The calcium acetate-strip system demonstrated a very high stability (Ea = 49.95 kcal/mol) to keep the spore viability and thermoresistance over steam cycles and storage. The heat activation kinetics of spores were also studied.
- Received June 7, 1995.
- Accepted November 1, 1995.
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