Abstract
E. coli strain HB101 was genetically engineered to a fluorescent phenotype by transformation with a plasmid containing complementary DNA for a green fluorescent protein. The level of fluorescence in the transformed strain was directly proportional to the number of viable cells. There was a rapid decrease in fluorescence when transformed cells were inoculated into lamivudine solutions containing ten different preservative formulations. The decrease in fluorescence correlated to a decrease in the number of viable cells, allowing the relative antimicrobial properties of each solution to be compared. This method provides a simple, rapid (<2 min./assay), and accurate means of determining the effects of antimicrobial solutions on the viability of E. coli.
- Received November 24, 1995.
- Accepted April 26, 1996.
- Copyright © Parenteral Drug Association. All rights reserved.
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