Reverse-Phase, High Performance Liquid Chromatographic Method for the Determination of Tolterodine Tartrate in Routine Quality Control Samples

V. S. N. Murthy Dwibhashyam; P. Keerthi; J. Vijaya Ratna; A. N. Nagappa

Abstract

A selective, sensitive, and rugged reverse phase-high performance liquid chromatographic method has been developed for the determination of tolterodine tartrate in routine quality control samples. The mobile phase consisted of acetonitrile:phosphate buffer (pH 7.0) in 55:45 v/v ratio. The mobile phase was also used for the extraction of tolterodine tartrate from its formulations. The chromatography was carried out on a Luna 100A, C-18 (5-μ, 250 × 4.60 mm) column. The software used in the chromatographic analysis was Empower Photodiode Array (PDA) software (Waters,™ Milford, CT). The UV spectrophotometric determination was done at 210 nm. Retention time was found to be about 7.0 ± 0.5 min. The standard curve was linear (r² = 0.9997) over the concentration range of 0.1–0.3 mg/mL. The method was found to be accurate, precise, specific, and rugged. The limit of detection was 0.16 μg/mL and the limit of quantification was 0.489 μg/mL. With a short chromatographic run time, the proposed method can be used for the estimation of large number of quality control samples in a short period.

Footnotes

View Full Text

PDA members receive access to all articles published in the current year and previous volume year. Institutional subscribers received access to all content. Log in below to receive access to this article if you are either of these.

If you are neither or you are a PDA member trying to access an article outside of your membership license, then you must purchase access to this article (below). If you do not have a username or password for JPST, you will be required to create an account prior to purchasing.

Full issue PDFs are for PDA members only.

Note to pda.org users

The PDA and PDA bookstore websites (www.pda.org and www.pda.org/bookstore) are separate websites from the PDA JPST website. When you first join PDA, your initial UserID and Password are sent to HighWirePress to create your PDA JPST account. Subsequent UserrID and Password changes required at the PDA websites will not pass on to PDA JPST and vice versa. If you forget your PDA JPST UserID and/or Password, you can request help to retrieve UserID and reset Password below.