Application of Flow Cytometry for Rapid Bioburden Screening in Vaccine Virus Production

Praful K. Bhusari; David E. Tabor; Ryan Yamagata; Mark S. Galinski

doi:10.5731/pdajpst.2012.00883

Research ArticleTechnology/Application

Application of Flow Cytometry for Rapid Bioburden Screening in Vaccine Virus Production

Praful K. Bhusari, David E. Tabor, Ryan Yamagata and Mark S. Galinski

PDA Journal of Pharmaceutical Science and Technology September 2012, 66 (5) 445-452; DOI: https://doi.org/10.5731/pdajpst.2012.00883

Praful K. Bhusari

MedImmune, LLC 319 North Bernardo Avenue, Mountain View, CA, USA

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For correspondence: bhusarip@Medimmune.com

David E. Tabor

MedImmune, LLC 319 North Bernardo Avenue, Mountain View, CA, USA

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Ryan Yamagata

MedImmune, LLC 319 North Bernardo Avenue, Mountain View, CA, USA

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Mark S. Galinski

MedImmune, LLC 319 North Bernardo Avenue, Mountain View, CA, USA

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vol. 66 no. 5 445-452

DOI

https://doi.org/10.5731/pdajpst.2012.00883

PubMed

23035028

Published By

Parenteral Drug Association (PDA)

Print ISSN

1079-7440

Online ISSN

1948-2124

History

Published online October 3, 2012.

Author Information

Praful K. Bhusari*,
David E. Tabor,
Ryan Yamagata and
Mark S. Galinski

MedImmune, LLC 319 North Bernardo Avenue, Mountain View, CA, USA

↵* Correspondence author: 650-603-2444. E-mail: bhusarip{at}Medimmune.com

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PDA Journal of Pharmaceutical Science and Technology

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Application of Flow Cytometry for Rapid Bioburden Screening in Vaccine Virus Production

Praful K. Bhusari, David E. Tabor, Ryan Yamagata, Mark S. Galinski

PDA Journal of Pharmaceutical Science and Technology Sep 2012, 66 (5) 445-452; DOI: 10.5731/pdajpst.2012.00883

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Application of Flow Cytometry for Rapid Bioburden Screening in Vaccine Virus Production

Praful K. Bhusari, David E. Tabor, Ryan Yamagata, Mark S. Galinski

PDA Journal of Pharmaceutical Science and Technology Sep 2012, 66 (5) 445-452; DOI: 10.5731/pdajpst.2012.00883

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Keywords

Rapid microbiology method
Presterile filtration bioburden
Flow cytometry
Viability staining
In-process control testing
Vaccine production
Microbiological sample preparation

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