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Research ArticleCONFERENCE PROCEEDING

Preliminary Evaluation of Next-Generation Sequencing Performance Relative to qPCR and In Vitro Cell Culture Tests for Human Cytomegalovirus

Siemon H.S. Ng, Ali Azizi, Kerrie Edamura, Rebecca J. Malott, Robert L. Charlebois, Carine Logvinoff, Martha Schreiber, Laurent Mallet and Lucy Gisonni-Lex
PDA Journal of Pharmaceutical Science and Technology November 2014, 68 (6) 563-571; DOI: https://doi.org/10.5731/pdajpst.2014.01013
Siemon H.S. Ng
1Microbiology & Virology Platform, Department of Analytical Research & Development North America, Sanofi Pasteur, Toronto, Ontario, Canada; and
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  • For correspondence: siemon.ng@sanofipasteur.com
Ali Azizi
1Microbiology & Virology Platform, Department of Analytical Research & Development North America, Sanofi Pasteur, Toronto, Ontario, Canada; and
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Kerrie Edamura
1Microbiology & Virology Platform, Department of Analytical Research & Development North America, Sanofi Pasteur, Toronto, Ontario, Canada; and
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Rebecca J. Malott
1Microbiology & Virology Platform, Department of Analytical Research & Development North America, Sanofi Pasteur, Toronto, Ontario, Canada; and
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Robert L. Charlebois
1Microbiology & Virology Platform, Department of Analytical Research & Development North America, Sanofi Pasteur, Toronto, Ontario, Canada; and
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Carine Logvinoff
1Microbiology & Virology Platform, Department of Analytical Research & Development North America, Sanofi Pasteur, Toronto, Ontario, Canada; and
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Martha Schreiber
1Microbiology & Virology Platform, Department of Analytical Research & Development North America, Sanofi Pasteur, Toronto, Ontario, Canada; and
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Laurent Mallet
2Product Conception and Development, Sanofi Pasteur, Marcy L'Etoile, France
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Lucy Gisonni-Lex
1Microbiology & Virology Platform, Department of Analytical Research & Development North America, Sanofi Pasteur, Toronto, Ontario, Canada; and
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PDA Journal of Pharmaceutical Science and Technology: 68 (6)
PDA Journal of Pharmaceutical Science and Technology
Vol. 68, Issue 6
November/December 2014
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Preliminary Evaluation of Next-Generation Sequencing Performance Relative to qPCR and In Vitro Cell Culture Tests for Human Cytomegalovirus
Siemon H.S. Ng, Ali Azizi, Kerrie Edamura, Rebecca J. Malott, Robert L. Charlebois, Carine Logvinoff, Martha Schreiber, Laurent Mallet, Lucy Gisonni-Lex
PDA Journal of Pharmaceutical Science and Technology Nov 2014, 68 (6) 563-571; DOI: 10.5731/pdajpst.2014.01013

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Preliminary Evaluation of Next-Generation Sequencing Performance Relative to qPCR and In Vitro Cell Culture Tests for Human Cytomegalovirus
Siemon H.S. Ng, Ali Azizi, Kerrie Edamura, Rebecca J. Malott, Robert L. Charlebois, Carine Logvinoff, Martha Schreiber, Laurent Mallet, Lucy Gisonni-Lex
PDA Journal of Pharmaceutical Science and Technology Nov 2014, 68 (6) 563-571; DOI: 10.5731/pdajpst.2014.01013
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Keywords

  • Next-generation sequencing
  • qPCR
  • Human cytomegalovirus
  • Illumina sequencing
  • Adventitious agent detection
  • HCMV
  • Herpesviridae

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