An Improved Method of Predicting Extinction Coefficients for the Determination of Protein Concentration

Eric C. Hilario; Alan Stern; Charlie H. Wang; Yenny W. Vargas; Charles J. Morgan; Trevor E. Swartz; Thomas W. Patapoff

doi:10.5731/pdajpst.2016.007120

Research ArticleTechnology/Application

An Improved Method of Predicting Extinction Coefficients for the Determination of Protein Concentration

Eric C. Hilario, Alan Stern, Charlie H. Wang, Yenny W. Vargas, Charles J. Morgan, Trevor E. Swartz and Thomas W. Patapoff

PDA Journal of Pharmaceutical Science and Technology March 2017, 71 (2) 127-135; DOI: https://doi.org/10.5731/pdajpst.2016.007120

Eric C. Hilario

1Early Stage Pharmaceutical Development,

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For correspondence: ehilario@gene.com

Alan Stern

2Late Stage Pharmaceutical Development,

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Charlie H. Wang

1Early Stage Pharmaceutical Development,

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Yenny W. Vargas

3Biostatistics Group, Genentech, Inc.,

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Charles J. Morgan

4Pharma Technical Regulatory, 1 DNA Way, South San Francisco, CA 94080, USA

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Trevor E. Swartz

1Early Stage Pharmaceutical Development,

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Thomas W. Patapoff

1Early Stage Pharmaceutical Development,

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vol. 71 no. 2 127-135

DOI

https://doi.org/10.5731/pdajpst.2016.007120

PubMed

27789804

Published By

Parenteral Drug Association (PDA)

Print ISSN

1079-7440

Online ISSN

1948-2124

History

Published online April 10, 2017.

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previous version (October 27, 2016 - 16:53).
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Author Information

Eric C. Hilario1,*,
Alan Stern2,
Charlie H. Wang1,
Yenny W. Vargas3,
Charles J. Morgan4,
Trevor E. Swartz1 and
Thomas W. Patapoff1

¹Early Stage Pharmaceutical Development,
²Late Stage Pharmaceutical Development,
³Biostatistics Group, Genentech, Inc.,
⁴Pharma Technical Regulatory, 1 DNA Way, South San Francisco, CA 94080, USA

↵* Corresponding Author: Eric Hilario, Genentech, Inc., 1 DNA Way, South San Francisco, CA 94080, USA. Telephone: 650-225-6693, e-mail: ehilario{at}gene.com

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An Improved Method of Predicting Extinction Coefficients for the Determination of Protein Concentration

Eric C. Hilario, Alan Stern, Charlie H. Wang, Yenny W. Vargas, Charles J. Morgan, Trevor E. Swartz, Thomas W. Patapoff

PDA Journal of Pharmaceutical Science and Technology Mar 2017, 71 (2) 127-135; DOI: 10.5731/pdajpst.2016.007120

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An Improved Method of Predicting Extinction Coefficients for the Determination of Protein Concentration

Eric C. Hilario, Alan Stern, Charlie H. Wang, Yenny W. Vargas, Charles J. Morgan, Trevor E. Swartz, Thomas W. Patapoff

PDA Journal of Pharmaceutical Science and Technology Mar 2017, 71 (2) 127-135; DOI: 10.5731/pdajpst.2016.007120

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Keywords

Extinction coefficient
Protein concentration
Absorbance spectrum
Absorptivity
Chromophores
Model compounds

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