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Direct detection of Burkholderia cepacia in susceptible pharmaceutical products using semi-nested PCR

Mohamed Attia, Amal Emad ElDin Ali, Tamer M Essam and Magdy Ali Amin
PDA Journal of Pharmaceutical Science and Technology January 2016, pdajpst.2015.006049; DOI: https://doi.org/10.5731/pdajpst.2015.006049
Mohamed Attia
1 Biotechnology Center, Faculty of Pharmacy, Cairo University;
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  • For correspondence: moh_a_attia@hotmail.com
Amal Emad ElDin Ali
2 Faculty of Pharmacy, Cairo University
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  • For correspondence: amal.ali@pharma.cu.edu.eg
Tamer M Essam
2 Faculty of Pharmacy, Cairo University
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  • For correspondence: tamer.essam@pharma.cu.edu.eg
Magdy Ali Amin
2 Faculty of Pharmacy, Cairo University
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  • For correspondence: magdy.amin@pharma.cu.edu.eg
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Article Information

pdajpst.2015.006049
DOI 
https://doi.org/10.5731/pdajpst.2015.006049
PubMed 
26797972

Published By 
Parenteral Drug Association (PDA)
Print ISSN 
1079-7440
Online ISSN 
1948-2124
History 
  • Received August 20, 2015
  • Accepted October 9, 2015
  • Published online January 21, 2016.

Article Versions

  • You are currently viewing a previous version of this article (January 21, 2016 - 17:53).
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Copyright & Usage 
Copyright © 2016, Parenteral Drug Association

Author Information

  1. Mohamed Attia1 (moh_a_attia{at}hotmail.com),
  2. Amal Emad ElDin Ali2,3 (amal.ali{at}pharma.cu.edu.eg),
  3. Tamer M Essam2 (tamer.essam{at}pharma.cu.edu.eg) and
  4. Magdy Ali Amin2 (magdy.amin{at}pharma.cu.edu.eg)
  1. 1 Biotechnology Center, Faculty of Pharmacy, Cairo University;
  2. 2 Faculty of Pharmacy, Cairo University
  1. ↵* Corresponding author; email: amal.ali{at}pharma.cu.edu.eg

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  • A comparison of culture-based, real-time PCR, droplet digital PCR and flow cytometric methods for the detection of Burkholderia cepacia complex in nuclease-free water and antiseptics
    Youngbeom Ahn, Bailey Gibson, Anna Williams, Pierre Alusta, Dan A Buzatu, Yong-Jin Lee, John J LiPuma, David Hussong, Bernard Marasa, Carl E Cerniglia
    Journal of Industrial Microbiology and Biotechnology 2020 47 6-7
  • A Propidium Monoazide (PMAxx)-Droplet Digital PCR (ddPCR) for the Detection of Viable Burkholderia cepacia Complex in Nuclease-Free Water and Antiseptics
    Soumana Daddy Gaoh, Ohgew Kweon, Yong-Jin Lee, David Hussong, Bernard Marasa, Youngbeom Ahn
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  • Loop-Mediated Isothermal Amplification (LAMP) Assay for Detecting Burkholderia cepacia Complex in Non-Sterile Pharmaceutical Products
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  • Specific Detection and Enumeration of Burkholderia cepacia Complex by Flow Cytometry Using a Fluorescence-Labeled Oligonucleotide Probe
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  • A culture-independent nucleic acid diagnostics method for use in the detection and quantification of Burkholderia cepacia complex contamination in aqueous finished pharmaceutical products
    Huong Duong, Elizabeth Minogue, Shannon Fullbrook, Thomas Barry, Kate Reddington, Annamaria Bevivino
    PLOS ONE 2024 19 5
  • Specific biomarker mining and rapid detection of Burkholderia cepacia complex by recombinase polymerase amplification
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    Frontiers in Microbiology 2023 14
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PDA Journal of Pharmaceutical Science and Technology: 79 (2)
PDA Journal of Pharmaceutical Science and Technology
Vol. 79, Issue 2
March/April 2025
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Direct detection of Burkholderia cepacia in susceptible pharmaceutical products using semi-nested PCR
Mohamed Attia, Amal Emad ElDin Ali, Tamer M Essam, Magdy Ali Amin
PDA Journal of Pharmaceutical Science and Technology Jan 2016, pdajpst.2015.006049; DOI: 10.5731/pdajpst.2015.006049

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Direct detection of Burkholderia cepacia in susceptible pharmaceutical products using semi-nested PCR
Mohamed Attia, Amal Emad ElDin Ali, Tamer M Essam, Magdy Ali Amin
PDA Journal of Pharmaceutical Science and Technology Jan 2016, pdajpst.2015.006049; DOI: 10.5731/pdajpst.2015.006049
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  • Identification of Burkholderia cepacia Complex by PCR: A Simple Way
  • Design, Development, and Validation of a Culture-Independent Nucleic Acid Diagnostics Method for the Rapid Detection and Quantification of the Burkholderia cepacia Complex in Water with an Equivalence to ISO/TS 12869:2019
  • Burkholderia cepacia Complex Bacteria: a Feared Contamination Risk in Water-Based Pharmaceutical Products
  • Real-Time PCR Detection of Burkholderia cepacia in Pharmaceutical Products Contaminated with Low Levels of Bacterial Contamination
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Keywords

  • Burkholderia cepacia
  • Non-sterile preparations
  • Syrup preparation
  • Vitek 2 compact system
  • conventional method
  • semi- nested PCR

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