Abstract
An important factor affecting the recovery of biological indicators (BI’s) is the time elapsed between exposure to a sterilant and growth testing. The length of time and the temperature at which the BI’s are stored after sterilization were found to significantly affect growth. BI’s stored at 20–25 °C for 48 hr prior to testing showed a 90% reduction in growth when compared to those that had been refrigerated at 2–8 °Cfor the same time period. This was consistent for B. subtilis and B. stcarothermophilus spores and independent of the type of sterilization process studied.
- Received April 3, 1980.
- Accepted June 6, 1980.
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