Reciprocal Translocation Observed in End-of-Production Cells of a Commercial CHO-Based Process

References

1. 1.↵
   1. Wuest D. M.,
   2. Harcum S. W.,
   3. Lee K. H.

   Genomics in mammalian cell culture bioprocessing. Biotechnol. Adv. 2012, 30 (3), 629–638.

   OpenUrlPubMed
2. 2.↵
   1. Barnes L. M.,
   2. Bentley C. M.,
   3. Dickson A. J.

   Stability of protein production from recombinant mammalian cells. Biotechnol Bioeng. 2003, 81 (6), 631–639.

   OpenUrlCrossRefPubMedWeb of Science
3. 3.↵
   1. Dorai H.,
   2. Corisdeo S.,
   3. Ellis D.,
   4. Kinney C.,
   5. Chomo M.,
   6. Hawley-Nelson P.,
   7. Moore G.,
   8. Betenbaugh M. J.,
   9. Ganguly S.

   Early prediction of instability of chinese hamster ovary cell lines expressing recombinant antibodies and antibody-fusion proteins. Biotechnol. Bioeng. 2012, 109 (4), 1016–1030.

   OpenUrlPubMed
4. 4.↵
   1. Chuck A. S.,
   2. Palsson B. O.

   Population balance between producing and nonproducing hybridoma clones is very sensitive to serum level, state of inoculum, and medium composition. Biotechnol. Bioeng. 1992, 39 (3), 354–360.

   OpenUrlPubMed
5. 5.↵
   1. Frame K. K.,
   2. Hu W. S.

   The loss of antibody productivity in continuous culture of hybridoma cells. Biotechnol. Bioeng. 1990, 35 (5), 469–476.

   OpenUrlCrossRefPubMed
6. 6.↵
   1. Dunn B. P.,
   2. Curtis J. R.

   Clastogenic agents in the urine of coffee drinkers and cigarette smokers. Mutat. Res. 1985, 147 (4), 179–188.

   OpenUrlPubMed
7. 7.↵
   1. Hacker D. L.,
   2. De Jesus M.,
   3. Wurm F. M.

   25 years of recombinant proteins from reactor-grown cells—Where do we go from here? Biotechnol. Adv. 2009, 27 (6), 1023–1027.

   OpenUrlCrossRefPubMed
8. 8.↵
   1. Jayapal K. P.,
   2. Wlaschin K. F.,
   3. Hu W. S.,
   4. Yap M. G. S.

   Recombinant protein therapeutics from CHO cells—20 years and counting. Chem. Enging. Prog. 2007, 103 (10), 40–47.

   OpenUrl
9. 9.↵
   1. Kim J. Y.,
   2. Kim Y. G.,
   3. Lee G. M.

   CHO cells in biotechnology for production of recombinant proteins: current state and further potential. Appl. Microbiol. Biotechnol. 2012, 93 (3), 917–930.

   OpenUrlCrossRefPubMedWeb of Science
10. 10.↵
    1. Wurm F. M.

    Production of recombinant protein therapeutics in cultivated mammalian cells. Nat. Biotechnol. 2004, 22 (11), 1393–1398.

    OpenUrlCrossRefPubMedWeb of Science
11. 11.↵
    1. Cao Y.,
    2. Kimura S.,
    3. Itoi T.,
    4. Honda K.,
    5. Ohtake H.,
    6. Omasa T.

    Fluorescence in situ hybridization using bacterial artificial chromosome (BAC) clones for the analysis of chromosome rearrangement in Chinese hamster ovary cells. Methods 2012, 56 (3), 418–423.

    OpenUrlPubMed
12. 12.↵
    1. Cao Y.,
    2. Kimura S.,
    3. Itoi T.,
    4. Honda K.,
    5. Ohtake H.,
    6. Omasa T.

    Construction of BAC-based physical map and analysis of chromosome rearrangement in Chinese hamster ovary cell lines. Biotechnol. Bioeng. 2012, 109 (6), 1357–1367.

    OpenUrlCrossRefPubMed
13. 13.↵
    1. Davies J.,
    2. Reff M.

    Chromosome localization and gene-copy-number quantification of three random integrations in Chinese-hamster ovary cells and their amplified cell lines using fluorescence in situ hybridization. Biotechnol. Appl. Biochem. 2001, 33 (2), 99–105.

    OpenUrlCrossRefPubMed
14. 14.↵
    1. Wurm F.

    CHO Quasispecies—implications for manufacturing processes. Processes 2013, 1 (3), 296–311.

    OpenUrl
15. 15.↵
    1. Derouazi M.,
    2. Martinet D.,
    3. Besuchet Schmutz N.,
    4. Flaction R.,
    5. Wicht M.,
    6. Bertschinger M.,
    7. Hacker D. L.,
    8. Beckmann J. S.,
    9. Wurm F. M.

    Genetic characterization of CHO production host DG44 and derivative recombinant cell lines. Biochem. Biophys. Res. Comm. 2006, 340 (4), 1069–1077.

    OpenUrlCrossRefPubMedWeb of Science
16. 16.↵
    1. Worton R. G.,
    2. Ho C. C.,
    3. Duff C.

    Chromosome stability in CHO cells. Somatic Cell Genet. 1977, 3 (1), 27–45.

    OpenUrlCrossRefPubMedWeb of Science
17. 17.↵
    1. Ruiz J. C.,
    2. Wahl G. M.

    Chromosomal destabilization during gene amplification. Mol. Cell. Biol. 1990, 10 (6), 3056–3066.

    OpenUrlAbstract/FREE Full Text
18. 18.↵
    1. Kim N. S.,
    2. Kim S. J.,
    3. Lee G. M.

    Clonal variability within dihydrofolate reductase-mediated gene amplified Chinese hamster ovary cells: stability in the absence of selective pressure. Biotechnol. Bioeng. 1998, 60 (6), 679–688.

    OpenUrlCrossRefPubMed
19. 19.↵
    1. Yoshikawa T.,
    2. Nakanishi F.,
    3. Ogura Y.,
    4. Oi D.,
    5. Omasa T.,
    6. Katakura Y.,
    7. Kishimoto M.,
    8. Suga K.

    Amplified gene location in chromosomal DNA affected recombinant protein production and stability of amplified genes. Biotechnol. Prog. 2000, 16 (5), 710–715.

    OpenUrlPubMed
20. 20.↵
    1. O'Callaghan P. M.,
    2. James D. C.

    Systems biotechnology of mammalian cell factories. Briefings in Functional Genomics and Proteomics 2008, 7 (2), 95–110.

    OpenUrlAbstract/FREE Full Text
21. 21.↵
    1. Fann C. H.,
    2. Guirgis F.,
    3. Chen G.,
    4. Lao M. S.,
    5. Piret J. M.

    Limitations to the amplification and stability of human tissue-type plasminogen activator expression by Chinese hamster ovary cells. Biotechnol. Bioeng. 2000, 69 (2), 204–212.

    OpenUrlPubMed
22. 22.↵
    1. Kim S. J.,
    2. Lee G. M.

    Cytogenetic analysis of chimeric antibody-producing CHO cells in the course of dihydrofolate reductase-mediated gene amplification and their stability in the absence of selective pressure. Biotechnol. Bioeng. 1999, 64 (6), 741–749.

    OpenUrlPubMed
23. 23.↵
    1. Xu X.,
    2. Nagarajan H.,
    3. Lewis N. E.,
    4. Pan S.,
    5. Cai Z.,
    6. Liu X.,
    7. Chen W.,
    8. Xie M.,
    9. Wang W.,
    10. Hammond S.,
    11. Andersen M. R.,
    12. Neff N.,
    13. Passarelli B.,
    14. Koh W.,
    15. Fan H. C.,
    16. Wang J.,
    17. Gui Y.,
    18. Lee K. H.,
    19. Betenbaugh M. J.,
    20. Quake S. R.,
    21. Famili I.,
    22. Palsson B. O.,
    23. Wang J.

    The genomic sequence of the Chinese hamster ovary (CHO)-K1 cell line. Nat. Biotechnol. 2011, 29 (8), 735–741.

    OpenUrlCrossRefPubMed
24. 24.↵
    1. Kantardjieff A.,
    2. Nissom P. M.,
    3. Chuah S. H.,
    4. Yusufi F.,
    5. Jacob N. M.,
    6. Mulukutla B. C.,
    7. Yap M.,
    8. Hu W. S.

    Developing genomic platforms for Chinese hamster ovary cells. Biotechnol. Adv. 2009, 27 (6), 1028–1035.

    OpenUrlPubMed
25. 25.↵
    1. Brinkrolf K.,
    2. Rupp O.,
    3. Laux H.,
    4. Kollin F.,
    5. Ernst W.,
    6. Linke B.,
    7. Kofler R.,
    8. Romand S.,
    9. Hesse F.,
    10. Budach W. E.,
    11. Galosy S.,
    12. Müller D.,
    13. Noll T.,
    14. Wienberg J.,
    15. Jostock T.,
    16. Leonard M.,
    17. Grillari J.,
    18. Tauch A.,
    19. Goesmann A.,
    20. Helk. B.,
    21. Mott J. E.,
    22. Pühler A.,
    23. Borth N.

    Chinese hamster genome sequenced from sorted chromosomes. Nat. Biotechnol. 2013, 31 (8), 694–695.

    OpenUrlPubMed
26. 26.↵
    1. Lewis N. E.,
    2. Liu X.,
    3. Li Y.,
    4. Nagarajan H.,
    5. Yerganian G.,
    6. O'Brien E.,
    7. Bordbar A.,
    8. Roth A. M.,
    9. Rosenbloom J.,
    10. Bian C.,
    11. Xie M.,
    12. Chen W.,
    13. Li N.,
    14. Baycin-Hizal D.,
    15. Latif H.,
    16. Forster J.,
    17. Betenbaugh M. J.,
    18. Famili I.,
    19. Xu X.,
    20. Wang J.,
    21. Palsson B. O.

    Genomic landscapes of Chinese hamster ovary cell lines as revealed by the Cricetulus griseus draft genome. Nat. Biotechnol. 2013, 31 (8), 759–765.

    OpenUrlCrossRefPubMed
27. 27.↵
    1. Hammill L.,
    2. Welles J.,
    3. Carson G. R.

    The gel microdrop secretion assay: Identification of a low productivity subpopulation arising during the production of human antibody in CHO cells. Cytotechnol. 2000, 34 (1–2), 27–37.

    OpenUrl
28. 28.↵
    1. Kim S. J.,
    2. Kim N. S.,
    3. Ryu C. J.,
    4. Hong H. J.,
    5. Lee G. M.

    Characterization of chimeric antibody producing CHO cells in the course of dihydrofolate reductase–mediated gene amplification and their stability in the absence of selective pressure. Biotechnol. Bioeng. 1998, 58 (1), 73–84.

    OpenUrlCrossRefPubMedWeb of Science
29. 29.↵
    1. Pallavicini M. G.,
    2. Deteresa P. S.,
    3. Rosette C.,
    4. Gray J. W.,
    5. Wurm F. M.

    Effects of methotrexate on transfected DNA stability in mammalian cells. Mol. Cell. Biol. 1990, 10 (1), 401–404.

    OpenUrlAbstract/FREE Full Text
30. 30.↵
    1. He L.,
    2. Winterrowd C.,
    3. Kadura I.,
    4. Frye C.

    Transgene copy number distribution profiles in recombinant CHO cell lines revealed by single cell analyses. Biotechnol. Bioengin. 2012, 109 (7), 1713–1722.

    OpenUrlPubMed
31. 31.↵
    1. Yoshikawa T.,
    2. Nakanishi F.,
    3. Itami S.,
    4. Kameoka D.,
    5. Omasa T.,
    6. Katakura Y.,
    7. Kishimoto M.,
    8. Suga K. I.

    Evaluation of stable and highly productive gene amplified CHO cell line based on the location of amplified genes. Cytotechnol. 2000, 33 (1–3), 37–46.

    OpenUrl
32. 32.↵
    1. Kilburn A. E.,
    2. Shea M. J.,
    3. Sargent R. G.,
    4. Wilson J. H.

    Insertion of a telomere repeat sequence into a mammalian gene causes chromosome instability. Mol. Cell. Biol. 2001, 21 (1), 126–135.

    OpenUrlAbstract/FREE Full Text
33. 33.↵
    1. Bolzán A. D.,
    2. Páez G. L.,
    3. Bianchi M. S.

    FISH analysis of telomeric repeat sequences and their involvement in chromosomal aberrations induced by radiomimetic compounds in hamster cells. Mutat. Res. 2001, 479 (1), 187–196.

    OpenUrlPubMedWeb of Science
34. 34.↵
    1. Chusainow J.,
    2. Yang Y. S.,
    3. Yeo J. H.,
    4. Toh P. C.,
    5. Asvadi P.,
    6. Wong N. S.,
    7. Yap M. G.

    A study of monoclonal antibody-producing CHO cell lines: What makes a stable high producer? Biotechnol. Bioeng. 2009, 102 (4), 1182–1196.

    OpenUrlCrossRefPubMed
35. 35.↵
    1. Pinkel D.,
    2. Straume T.,
    3. Gray J. W.

    Cytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridization. Proc. Natl. Acad. Sci. of the U.S.A. 1986, 83 (9), 2934–2938.

    OpenUrl
36. 36.↵
    1. Ried T.,
    2. Schröck E.,
    3. Ning Y.,
    4. Wienberg J.

    Chromosome painting: a useful art. Hum. Mol. Genet. 1998, 7 (10), 1619–1626.

    OpenUrlAbstract/FREE Full Text
37. 37.↵
    1. Speicher M. R.,
    2. Carter N. P.

    The new cytogenetics: blurring the boundaries with molecular biology. Nat. Rev. Genet. 2005, 6 (10), 782–792.

    OpenUrlCrossRefPubMedWeb of Science
38. 38.↵
    1. Ishidate M. Jr..,
    2. Harnois M. C.,
    3. Sofuni T.

    A comparative analysis of data on the clastogenicity of 951 chemical substances tested in mammalian cell cultures. Mutat. Res. 1988, 195 (2), 151–213.

    OpenUrlPubMedWeb of Science
39. 39.↵
    1. Witte I.,
    2. Plappert U.,
    3. de Wall H.,
    4. Hartmann A.

    Genetic toxicity assessment: employing the best science for human safety evaluation. Part III: The comet assay as an alternative to in vitro clastogenicity tests for early drug candidate selection. Toxicol. Sci. 2007, 97 (1), 21–26.

    OpenUrlAbstract/FREE Full Text
40. 40.↵
    1. Flintoff W. F.,
    2. Livingston E.,
    3. Duff C.,
    4. Worton R. G.

    Moderate-level gene amplification in methotrexate-resistant Chinese hamster ovary cells is accompanied by chromosomal translocations at or near the site of the amplified DHFR gene. Mol. Cell. Biol. 1984, 4 (1), 69–76.

    OpenUrlAbstract/FREE Full Text