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Research ArticleResearch

Direct Detection of Burkholderia cepacia in Susceptible Pharmaceutical Products Using Semi-Nested PCR

Mohamed A. Attia, Amal E. Ali, Tamer M. Essam and Magdy A. Amin
PDA Journal of Pharmaceutical Science and Technology March 2016, 70 (2) 99-108; DOI: https://doi.org/10.5731/pdajpst.2015.006049
Mohamed A. Attia
1Biotechnology Center, Faculty of Pharmacy, Cairo University, Cairo, Egypt;
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Amal E. Ali
2Microbiology and Immunology Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt; and
3Microbiology and Immunology Department, Faculty of Pharmaceutical Sciences & Pharmaceutical Industries, Future University in Egypt, New Cairo, Egypt
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  • For correspondence: amal.ali@pharma.cu.edu.eg amal.emad@fue.edu.eg
Tamer M. Essam
1Biotechnology Center, Faculty of Pharmacy, Cairo University, Cairo, Egypt;
2Microbiology and Immunology Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt; and
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Magdy A. Amin
2Microbiology and Immunology Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt; and
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Article Information

vol. 70 no. 2 99-108
DOI 
https://doi.org/10.5731/pdajpst.2015.006049
PubMed 
26797972

Published By 
Parenteral Drug Association (PDA)
Print ISSN 
1079-7440
Online ISSN 
1948-2124
History 
  • Published online April 13, 2016.

Article Versions

  • previous version (January 21, 2016 - 17:53).
  • You are viewing the most recent version of this article.
Copyright & Usage 
© PDA, Inc. 2016

Author Information

  1. Mohamed A. Attia1,
  2. Amal E. Ali2,3,*,
  3. Tamer M. Essam1,2 and
  4. Magdy A. Amin2
  1. 1Biotechnology Center, Faculty of Pharmacy, Cairo University, Cairo, Egypt;
  2. 2Microbiology and Immunology Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt; and
  3. 3Microbiology and Immunology Department, Faculty of Pharmaceutical Sciences & Pharmaceutical Industries, Future University in Egypt, New Cairo, Egypt
  1. ↵* Corresponding Author: Dr. Amal E. Ali, Faculty of Pharmacy, Cairo University, Department of Microbiology and Immunology, Kasr Eleini Street. Cairo, 11562, Egypt. Telephone number: +2-02-25353100/200/300/400; Mobile: +20100-8551561; e-mail: amal.ali{at}pharma.cu.edu.eg; amal.emad{at}fue.edu.eg
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  • A comparison of culture-based, real-time PCR, droplet digital PCR and flow cytometric methods for the detection of Burkholderia cepacia complex in nuclease-free water and antiseptics
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  • A culture-independent nucleic acid diagnostics method for use in the detection and quantification of Burkholderia cepacia complex contamination in aqueous finished pharmaceutical products
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  • Specific biomarker mining and rapid detection of Burkholderia cepacia complex by recombinase polymerase amplification
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PDA Journal of Pharmaceutical Science and Technology: 70 (2)
PDA Journal of Pharmaceutical Science and Technology
Vol. 70, Issue 2
March/April 2016
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Direct Detection of Burkholderia cepacia in Susceptible Pharmaceutical Products Using Semi-Nested PCR
Mohamed A. Attia, Amal E. Ali, Tamer M. Essam, Magdy A. Amin
PDA Journal of Pharmaceutical Science and Technology Mar 2016, 70 (2) 99-108; DOI: 10.5731/pdajpst.2015.006049

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Direct Detection of Burkholderia cepacia in Susceptible Pharmaceutical Products Using Semi-Nested PCR
Mohamed A. Attia, Amal E. Ali, Tamer M. Essam, Magdy A. Amin
PDA Journal of Pharmaceutical Science and Technology Mar 2016, 70 (2) 99-108; DOI: 10.5731/pdajpst.2015.006049
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Cited By...

  • Identification of Burkholderia cepacia Complex by PCR: A Simple Way
  • Design, Development, and Validation of a Culture-Independent Nucleic Acid Diagnostics Method for the Rapid Detection and Quantification of the Burkholderia cepacia Complex in Water with an Equivalence to ISO/TS 12869:2019
  • Burkholderia cepacia Complex Bacteria: a Feared Contamination Risk in Water-Based Pharmaceutical Products
  • Real-Time PCR Detection of Burkholderia cepacia in Pharmaceutical Products Contaminated with Low Levels of Bacterial Contamination
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Keywords

  • B. cepacia
  • Susceptible pharmaceutical products
  • Semi-nested polymerase chain reaction
  • conventional culturing
  • Biochemical methods

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