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Research ArticleResearch

Direct Detection of Burkholderia cepacia in Susceptible Pharmaceutical Products Using Semi-Nested PCR

Mohamed A. Attia, Amal E. Ali, Tamer M. Essam and Magdy A. Amin
PDA Journal of Pharmaceutical Science and Technology March 2016, 70 (2) 99-108; DOI: https://doi.org/10.5731/pdajpst.2015.006049
Mohamed A. Attia
1Biotechnology Center, Faculty of Pharmacy, Cairo University, Cairo, Egypt;
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Amal E. Ali
2Microbiology and Immunology Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt; and
3Microbiology and Immunology Department, Faculty of Pharmaceutical Sciences & Pharmaceutical Industries, Future University in Egypt, New Cairo, Egypt
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  • For correspondence: amal.ali@pharma.cu.edu.eg amal.emad@fue.edu.eg
Tamer M. Essam
1Biotechnology Center, Faculty of Pharmacy, Cairo University, Cairo, Egypt;
2Microbiology and Immunology Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt; and
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Magdy A. Amin
2Microbiology and Immunology Department, Faculty of Pharmacy, Cairo University, Cairo, Egypt; and
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PDA Journal of Pharmaceutical Science and Technology: 70 (2)
PDA Journal of Pharmaceutical Science and Technology
Vol. 70, Issue 2
March/April 2016
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Direct Detection of Burkholderia cepacia in Susceptible Pharmaceutical Products Using Semi-Nested PCR
Mohamed A. Attia, Amal E. Ali, Tamer M. Essam, Magdy A. Amin
PDA Journal of Pharmaceutical Science and Technology Mar 2016, 70 (2) 99-108; DOI: 10.5731/pdajpst.2015.006049

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Direct Detection of Burkholderia cepacia in Susceptible Pharmaceutical Products Using Semi-Nested PCR
Mohamed A. Attia, Amal E. Ali, Tamer M. Essam, Magdy A. Amin
PDA Journal of Pharmaceutical Science and Technology Mar 2016, 70 (2) 99-108; DOI: 10.5731/pdajpst.2015.006049
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    • 1. Introduction
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  • Identification of Burkholderia cepacia Complex by PCR: A Simple Way
  • Design, Development, and Validation of a Culture-Independent Nucleic Acid Diagnostics Method for the Rapid Detection and Quantification of the Burkholderia cepacia Complex in Water with an Equivalence to ISO/TS 12869:2019
  • Burkholderia cepacia Complex Bacteria: a Feared Contamination Risk in Water-Based Pharmaceutical Products
  • Real-Time PCR Detection of Burkholderia cepacia in Pharmaceutical Products Contaminated with Low Levels of Bacterial Contamination
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Keywords

  • B. cepacia
  • Susceptible pharmaceutical products
  • Semi-nested polymerase chain reaction
  • conventional culturing
  • Biochemical methods

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