Quantifying Operator Subjectivity within Flow Cytometry Data Analysis as a Source of Measurement Uncertainty and the Impact of Experience on Results

Rebecca Grant; Karen Coopman; Nicholas Medcalf; Sandro Silva-Gomes; Jonathan J. Campbell; Bo Kara; Julian Braybrook; Jon Petzing

doi:10.5731/pdajpst.2019.011213

Research ArticleResearch

Quantifying Operator Subjectivity within Flow Cytometry Data Analysis as a Source of Measurement Uncertainty and the Impact of Experience on Results

Rebecca Grant, Karen Coopman, Nicholas Medcalf, Sandro Silva-Gomes, Jonathan J. Campbell, Bo Kara, Julian Braybrook and Jon Petzing

PDA Journal of Pharmaceutical Science and Technology January 2021, 75 (1) 33-47; DOI: https://doi.org/10.5731/pdajpst.2019.011213

Rebecca Grant

1Wolfson School of Mechanical, Electrical and Manufacturing Engineering, Loughborough University, Leicestershire, LE11 3TU, United Kingdom;

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For correspondence: r.grant@lboro.ac.uk

Karen Coopman

2Chemical Engineering, Loughborough University, Leicestershire, LE11 3TU, United Kingdom;

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Nicholas Medcalf

1Wolfson School of Mechanical, Electrical and Manufacturing Engineering, Loughborough University, Leicestershire, LE11 3TU, United Kingdom;

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Sandro Silva-Gomes

3GlaxoSmithKline, Gunnels Wood Road, Stevenage, United Kingdom; and

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Jonathan J. Campbell

4LGC, Queen’s Road, Teddington, Middlesex, TW11 0LY, United Kingdom

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Bo Kara

3GlaxoSmithKline, Gunnels Wood Road, Stevenage, United Kingdom; and

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Julian Braybrook

4LGC, Queen’s Road, Teddington, Middlesex, TW11 0LY, United Kingdom

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Jon Petzing

1Wolfson School of Mechanical, Electrical and Manufacturing Engineering, Loughborough University, Leicestershire, LE11 3TU, United Kingdom;

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vol. 75 no. 1 33-47

DOI

https://doi.org/10.5731/pdajpst.2019.011213

PubMed

33067330

Published By

Parenteral Drug Association (PDA)

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1079-7440

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1948-2124

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Published online February 22, 2021.

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previous version (October 16, 2020 - 09:14).
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Author Information

Rebecca Grant^1,*,
Karen Coopman²,
Nicholas Medcalf¹,
Sandro Silva-Gomes³,
Jonathan J. Campbell⁴,
Bo Kara³,
Julian Braybrook⁴ and
Jon Petzing¹

¹Wolfson School of Mechanical, Electrical and Manufacturing Engineering, Loughborough University, Leicestershire, LE11 3TU, United Kingdom;
²Chemical Engineering, Loughborough University, Leicestershire, LE11 3TU, United Kingdom;
³GlaxoSmithKline, Gunnels Wood Road, Stevenage, United Kingdom; and
⁴LGC, Queen’s Road, Teddington, Middlesex, TW11 0LY, United Kingdom

↵* Corresponding Author: Wolfson School of Mechanical, Electrical and Manufacturing Engineering, Loughborough University, Leicestershire, LE11 3TU, United Kingdom; E-mail: r.grant{at}lboro.ac.uk

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Quantifying Operator Subjectivity within Flow Cytometry Data Analysis as a Source of Measurement Uncertainty and the Impact of Experience on Results

Rebecca Grant, Karen Coopman, Nicholas Medcalf, Sandro Silva-Gomes, Jonathan J. Campbell, Bo Kara, Julian Braybrook, Jon Petzing

PDA Journal of Pharmaceutical Science and Technology Jan 2021, 75 (1) 33-47; DOI: 10.5731/pdajpst.2019.011213

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Quantifying Operator Subjectivity within Flow Cytometry Data Analysis as a Source of Measurement Uncertainty and the Impact of Experience on Results

Rebecca Grant, Karen Coopman, Nicholas Medcalf, Sandro Silva-Gomes, Jonathan J. Campbell, Bo Kara, Julian Braybrook, Jon Petzing

PDA Journal of Pharmaceutical Science and Technology Jan 2021, 75 (1) 33-47; DOI: 10.5731/pdajpst.2019.011213

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Keywords

Flow cytometry
Variation
Operators
Gauge R&R
Uncertainties
Measurement uncertainty

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