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Research ArticleResearch

Quantifying Operator Subjectivity within Flow Cytometry Data Analysis as a Source of Measurement Uncertainty and the Impact of Experience on Results

Rebecca Grant, Karen Coopman, Nicholas Medcalf, Sandro Silva-Gomes, Jonathan J. Campbell, Bo Kara, Julian Braybrook and Jon Petzing
PDA Journal of Pharmaceutical Science and Technology January 2021, 75 (1) 33-47; DOI: https://doi.org/10.5731/pdajpst.2019.011213
Rebecca Grant
1Wolfson School of Mechanical, Electrical and Manufacturing Engineering, Loughborough University, Leicestershire, LE11 3TU, United Kingdom;
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  • For correspondence: r.grant@lboro.ac.uk
Karen Coopman
2Chemical Engineering, Loughborough University, Leicestershire, LE11 3TU, United Kingdom;
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Nicholas Medcalf
1Wolfson School of Mechanical, Electrical and Manufacturing Engineering, Loughborough University, Leicestershire, LE11 3TU, United Kingdom;
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Sandro Silva-Gomes
3GlaxoSmithKline, Gunnels Wood Road, Stevenage, United Kingdom; and
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Jonathan J. Campbell
4LGC, Queen’s Road, Teddington, Middlesex, TW11 0LY, United Kingdom
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Bo Kara
3GlaxoSmithKline, Gunnels Wood Road, Stevenage, United Kingdom; and
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Julian Braybrook
4LGC, Queen’s Road, Teddington, Middlesex, TW11 0LY, United Kingdom
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Jon Petzing
1Wolfson School of Mechanical, Electrical and Manufacturing Engineering, Loughborough University, Leicestershire, LE11 3TU, United Kingdom;
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Article Information

vol. 75 no. 1 33-47
DOI 
https://doi.org/10.5731/pdajpst.2019.011213
PubMed 
33067330

Published By 
Parenteral Drug Association (PDA)
Print ISSN 
1079-7440
Online ISSN 
1948-2124
History 
  • Published online February 22, 2021.

Article Versions

  • previous version (October 16, 2020 - 09:14).
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Copyright & Usage 
© PDA, Inc. 2021

Author Information

  1. Rebecca Grant1,*,
  2. Karen Coopman2,
  3. Nicholas Medcalf1,
  4. Sandro Silva-Gomes3,
  5. Jonathan J. Campbell4,
  6. Bo Kara3,
  7. Julian Braybrook4 and
  8. Jon Petzing1
  1. 1Wolfson School of Mechanical, Electrical and Manufacturing Engineering, Loughborough University, Leicestershire, LE11 3TU, United Kingdom;
  2. 2Chemical Engineering, Loughborough University, Leicestershire, LE11 3TU, United Kingdom;
  3. 3GlaxoSmithKline, Gunnels Wood Road, Stevenage, United Kingdom; and
  4. 4LGC, Queen’s Road, Teddington, Middlesex, TW11 0LY, United Kingdom
  1. ↵* Corresponding Author: Wolfson School of Mechanical, Electrical and Manufacturing Engineering, Loughborough University, Leicestershire, LE11 3TU, United Kingdom; E-mail: r.grant{at}lboro.ac.uk
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PDA Journal of Pharmaceutical Science and Technology: 75 (1)
PDA Journal of Pharmaceutical Science and Technology
Vol. 75, Issue 1
January/February 2021
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Quantifying Operator Subjectivity within Flow Cytometry Data Analysis as a Source of Measurement Uncertainty and the Impact of Experience on Results
Rebecca Grant, Karen Coopman, Nicholas Medcalf, Sandro Silva-Gomes, Jonathan J. Campbell, Bo Kara, Julian Braybrook, Jon Petzing
PDA Journal of Pharmaceutical Science and Technology Jan 2021, 75 (1) 33-47; DOI: 10.5731/pdajpst.2019.011213

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Quantifying Operator Subjectivity within Flow Cytometry Data Analysis as a Source of Measurement Uncertainty and the Impact of Experience on Results
Rebecca Grant, Karen Coopman, Nicholas Medcalf, Sandro Silva-Gomes, Jonathan J. Campbell, Bo Kara, Julian Braybrook, Jon Petzing
PDA Journal of Pharmaceutical Science and Technology Jan 2021, 75 (1) 33-47; DOI: 10.5731/pdajpst.2019.011213
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Keywords

  • Flow cytometry
  • Variation
  • Operators
  • Gauge R&R
  • Uncertainties
  • Measurement uncertainty

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