Investigating Tm Method Specificity Using Oligonucleotide Sequence Variants

Alexandra H. Heussner; Sarina Schuler; Gerd Berger; Melanie Zerulla-Wernitz

doi:10.5731/pdajpst.2021.012694

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Investigating T_m Method Specificity Using Oligonucleotide Sequence Variants

Alexandra H. Heussner, Sarina Schuler, Gerd Berger and Melanie Zerulla-Wernitz

PDA Journal of Pharmaceutical Science and Technology March 2023, 77 (2) 67-78; DOI: https://doi.org/10.5731/pdajpst.2021.012694

Alexandra H. Heussner

Analytical Science Laboratory, Development Service, Vetter Pharma-Fertigung GmbH & Co. KG, 88212 Ravensburg, Germany

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For correspondence: alexandra.heussner@vetter-pharma.com

Sarina Schuler

Analytical Science Laboratory, Development Service, Vetter Pharma-Fertigung GmbH & Co. KG, 88212 Ravensburg, Germany

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Gerd Berger

Analytical Science Laboratory, Development Service, Vetter Pharma-Fertigung GmbH & Co. KG, 88212 Ravensburg, Germany

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Melanie Zerulla-Wernitz

Analytical Science Laboratory, Development Service, Vetter Pharma-Fertigung GmbH & Co. KG, 88212 Ravensburg, Germany

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vol. 77 no. 2 67-78

DOI

https://doi.org/10.5731/pdajpst.2021.012694

PubMed

36122915

Published By

Parenteral Drug Association (PDA)

Print ISSN

1079-7440

Online ISSN

1948-2124

History

Published online April 10, 2023.

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previous version (September 19, 2022 - 04:02).
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Alexandra H. Heussner*,
Sarina Schuler,
Gerd Berger and
Melanie Zerulla-Wernitz

Analytical Science Laboratory, Development Service, Vetter Pharma-Fertigung GmbH & Co. KG, 88212 Ravensburg, Germany

↵*Corresponding Author: Analytical Science Laboratory, Development Service, Vetter Pharma-Fertigung GmbH & Co. KG, Schuetzenstrasse 87, 88212 Ravensburg, Germany; E-mail: alexandra.heussner{at}vetter-pharma.com

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Investigating T_m Method Specificity Using Oligonucleotide Sequence Variants

Alexandra H. Heussner, Sarina Schuler, Gerd Berger, Melanie Zerulla-Wernitz

PDA Journal of Pharmaceutical Science and Technology Mar 2023, 77 (2) 67-78; DOI: 10.5731/pdajpst.2021.012694

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Investigating T_m Method Specificity Using Oligonucleotide Sequence Variants

Alexandra H. Heussner, Sarina Schuler, Gerd Berger, Melanie Zerulla-Wernitz

PDA Journal of Pharmaceutical Science and Technology Mar 2023, 77 (2) 67-78; DOI: 10.5731/pdajpst.2021.012694

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Keywords

Oligonucleotide
Identity
Specificity
Melting temperature
Tm
Hyperchromicity
quality control

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