Abstract
Multilamellar and unilamellar liposomes containing sodium methotrexate (MTX) were prepared by thin film hydration and reverse-phase evaporation methods. Encapsulation efficiency and release of MTX from liposomes at 4°C, 25°C and 37°C were determined. When stored at 25°C the liposomes lost 33% MTX after one month. To stabilize liposomes, they were freeze-dried in presence of different amounts of cryoprotectants (trehalose, PEG 10,000, PEG 20,000, and a mixture of trehalose-PEG 20,000). Measurements showed that the mixture of trehalose-PEG 20,000 imparted an MTX retention of about 70% after lyophilization. Stability tests of the lyophilized product at 4°C, 25°C and 37°C demonstrated they were fairly stable even at 37°C, indicating their potential use in commercial applications.
Footnotes
- Received March 14, 1997.
- Accepted September 12, 1997.
- Copyright © Parenteral Drug Association. All rights reserved.
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