Preparation and Stability of Diallyl Trisulfide Self-Assembled Micellar Injection

Xiulan Ju; Shixuan Zhang; Qing Wang; Xiaona Li; Puwen Yang

Abstract

Diallyl trisulfide (DATS) has shown various biological activities, such as antimicrobial, antihyperlipidemic, antithrombotic, and anticancer effects. It is a highly water insoluble thioether compound, which irritates blood vessels and degrades rapidly under normal conditions. Hence, a suitable DATS delivery system and the related stability studies are both valuable for the clinical use of DATS. The DATS micellar injection in the present study was prepared using propylene glycol, alcohol, Tween 80, and water by the self-assembling micellar method, which effectively avoided irritation and degradation of DATS. The high-pressure liquid chromatography-UV assay method was established and validated for the quantitative determination of DATS micellar injection. The major degraded compounds were identified as diallyl disulfide and diallyl tetrasulfide by gas chromatography–mass spectrometry. 3-ethenyl-3,6-dihydro-1,2-dithiin and 3-ethenyl-3,4-dihydro-1,2-dithiin might be the thermal decomposed products of DATS in process of gas chromatography–flame ionization detector analysis. The main factor influencing stability in DATS micellar injection was ascertained to be temperature. This investigation might be a beneficial contribution to further identification of DATS-degraded products and exploration of the delivery system of DATS.

Footnotes

Declaration of interest: The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.

View Full Text

PDA members receive access to all articles published in the current year and previous volume year. Institutional subscribers received access to all content. Log in below to receive access to this article if you are either of these.

If you are neither or you are a PDA member trying to access an article outside of your membership license, then you must purchase access to this article (below). If you do not have a username or password for JPST, you will be required to create an account prior to purchasing.

Full issue PDFs are for PDA members only.

Note to pda.org users

The PDA and PDA bookstore websites (www.pda.org and www.pda.org/bookstore) are separate websites from the PDA JPST website. When you first join PDA, your initial UserID and Password are sent to HighWirePress to create your PDA JPST account. Subsequent UserrID and Password changes required at the PDA websites will not pass on to PDA JPST and vice versa. If you forget your PDA JPST UserID and/or Password, you can request help to retrieve UserID and reset Password below.