Abstract
Memberane Filtration and epiflurescence microscopy were combined with a sample incubation stage to enable the detection of low levels of bacterial contaminantes.The device and technique described allows the detection of as few as six viable organisms within a total incubation and analysis time of 6 hr,irrespective of sample volume.Linear relationships (corrlation coeficients ≥ 0.940)were shown to exist between post-incubation membrane fluorescence counts and initial pour plate colony counts for both vegetation and spore-forming bacteria and for all incubation times tested. The technique was successfully applied to the detection and enumeration of a mixed flora in normal saline and of survivours in a phenol-preserved.solution
- Received November 17, 1986.
- Accepted January 10, 1987.
- Copyright © Parenteral Drug Association. All rights reserved.
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